ZFIN ID: ZDB-FIG-101206-4
Mahmoudi et al., 2010 - The Leukemia-Associated Mllt10/Af10-Dot1l Are Tcf4/{beta}-Catenin Coactivators Essential for Intestinal Homeostasis. PLoS Biology   8(11):e1000539 Full text @ PLoS Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. 9 tcf7l2, mllt10/af10, and dot1l are essential for Wnt target gene expression and intestinal homeostasis in zebrafish.
(A–H) Representative image of BrdU incorporation into epithelial cells in the intestinal bulb of 124 hpf zebrafish embryos. BrdU staining in wild-type embryos (A) and embryos depleted of tcf7l2 (B), mllt10/af10 (C,D), and dot1l (E,F), p53 (G), and control (H) by MO injection. (I) Quantification (mean ± SD) of percent BrdU positive cells and (J) total number of cells in the epithelial layer of the intestinal bulb (20 embryos/condition). * p<0.05. Depletion of tcf7l2, mllt10/af10, and dot1l MO abrogates axin2 expression in intestine. In situ hybridization for axin2 in 80 hpf wild-type and MO-injected embryos (K–R). Staining for axin2 in wild type embryos uninjected (K) or injected with MO against tcf7l2 (L), mllt10/af10 (M,N), and dot1l (O,P), p53 (Q), and control MO (R). All MOs have been coinjected with a MO against p53. In parentheses number of embryos with phenotype per total analyzed. Images are representative of three independent experiments. sb, swim bladder; ib, intestinal bulb. Scale bar: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
axin2 WT control Protruding-mouth intestinal epithelium ISH
WT + MO1-dot1l + MO4-tp53 standard conditions Protruding-mouth Not Detected intestinal epithelium ISH
WT + MO1-mllt10 + MO4-tp53 standard conditions Protruding-mouth Not Detected intestinal epithelium ISH
WT + MO2-dot1l + MO4-tp53 standard conditions Protruding-mouth Not Detected intestinal epithelium ISH
WT + MO2-mllt10 + MO4-tp53 standard conditions Protruding-mouth Not Detected intestinal epithelium ISH
WT + MO3-tcf7l2 + MO4-tp53 standard conditions Protruding-mouth Not Detected intestinal epithelium ISH
WT + MO4-tp53 standard conditions Protruding-mouth intestinal epithelium ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-dot1l + MO4-tp53 standard conditions Protruding-mouth canonical Wnt signaling pathway decreased process quality, abnormal
Day 5 intestinal epithelial structure maintenance decreased process quality, abnormal
Day 5 intestinal epithelium has fewer parts of type epithelial cell, abnormal
Day 5 intestinal villus decreased length, abnormal
WT + MO1-mllt10 + MO4-tp53 standard conditions Protruding-mouth canonical Wnt signaling pathway decreased process quality, abnormal
Day 5 intestinal epithelial structure maintenance decreased process quality, abnormal
Day 5 intestinal epithelium has fewer parts of type epithelial cell, abnormal
Day 5 intestinal villus decreased length, abnormal
WT + MO2-dot1l + MO4-tp53 standard conditions Protruding-mouth canonical Wnt signaling pathway decreased process quality, abnormal
Day 5 intestinal epithelial structure maintenance decreased process quality, abnormal
Day 5 intestinal epithelium has fewer parts of type epithelial cell, abnormal
Day 5 intestinal villus decreased length, abnormal
WT + MO2-mllt10 + MO4-tp53 standard conditions Protruding-mouth canonical Wnt signaling pathway decreased process quality, abnormal
Day 5 intestinal epithelial structure maintenance decreased process quality, abnormal
Day 5 intestinal epithelium has fewer parts of type epithelial cell, abnormal
Day 5 intestinal villus decreased length, abnormal
WT + MO3-tcf7l2 + MO4-tp53 standard conditions Protruding-mouth canonical Wnt signaling pathway decreased process quality, abnormal
Day 5 intestinal epithelial structure maintenance decreased process quality, abnormal
Day 5 intestinal epithelium has fewer parts of type epithelial cell, abnormal
Day 5 intestinal villus decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Biol.