tcf7l2, mllt10/af10, and dot1l are essential for Wnt target gene expression and intestinal homeostasis in zebrafish.
(A–H) Representative image of BrdU incorporation into epithelial cells in the intestinal bulb of 124 hpf zebrafish embryos. BrdU staining in wild-type embryos (A) and embryos depleted of tcf7l2 (B), mllt10/af10 (C,D), and dot1l (E,F), p53 (G), and control (H) by MO injection. (I) Quantification (mean ± SD) of percent BrdU positive cells and (J) total number of cells in the epithelial layer of the intestinal bulb (20 embryos/condition). * p<0.05. Depletion of tcf7l2, mllt10/af10, and dot1l MO abrogates axin2 expression in intestine. In situ hybridization for axin2 in 80 hpf wild-type and MO-injected embryos (K–R). Staining for axin2 in wild type embryos uninjected (K) or injected with MO against tcf7l2 (L), mllt10/af10 (M,N), and dot1l (O,P), p53 (Q), and control MO (R). All MOs have been coinjected with a MO against p53. In parentheses number of embryos with phenotype per total analyzed. Images are representative of three independent experiments. sb, swim bladder; ib, intestinal bulb. Scale bar: 100 μm.