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ZFIN ID: ZDB-FIG-101206-3
Mahmoudi et al., 2010 - The Leukemia-Associated Mllt10/Af10-Dot1l Are Tcf4/{beta}-Catenin Coactivators Essential for Intestinal Homeostasis. PLoS Biology   8(11):e1000539 Full text @ PLoS Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 8 Depletion of mllt10/af10 and dot1l rescues intestinal defects in apcmcr/mcr zebrafish, mimicking tcf7l2 depletion.
Depletion of mllt10/af10 and dot1l rescues expression of i-fabp in apcmcr/mcr mutant embryos, placing these genes downstream of Apc as Wnt target gene activators (A–N). Representative whole mount in situ hybridizations for i-fabp in wild type and apcmcr/mcr mutant embryos at 80 hpf injected with (A,B) buffer alone,(C,D) MO against tcf7l2, (E–H) two independent mllt10/af10 MOs, (I–L) two independent dot1l MO, and (M,N) control MO. Depletion of mllt10/af10 and dot1l rescues mis-expression of cyp26a1 in apcmcr/mcr mutant embryos (O–AB). Representative whole mount in situ hybridizations for cyp26a1 in wild type and apcmcr/mcr mutant embryos at 80 hpf injected with (O,P) buffer alone, (Q,R) MO against tcf7l2, (S–V) two independent mllt10/af10 MOs, (W–Z) two independent dot1l MO, and (AA,AB) control MO. All MOs have been coinjected with an MO against p53. All images were captured using the same exposure and represent at least three independent experiments. In parentheses number of embryos showing described phenotype per number of total embryos analyzed.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
fabp2 WT control Protruding-mouth intestine ISH
WT + MO1-dot1l + MO4-tp53 standard conditions Protruding-mouth intestine ISH
WT + MO1-mllt10 + MO4-tp53 standard conditions Protruding-mouth intestine ISH
WT + MO2-dot1l + MO4-tp53 standard conditions Protruding-mouth intestine ISH
WT + MO2-mllt10 + MO4-tp53 standard conditions Protruding-mouth intestine ISH
WT + MO3-tcf7l2 + MO4-tp53 standard conditions Protruding-mouth intestine ISH
WT + MO4-tp53 standard conditions Protruding-mouth intestine ISH
apchu745/hu745 control Protruding-mouth Not Detected intestine ISH
apchu745/hu745 + MO1-dot1l + MO4-tp53 standard conditions Protruding-mouth intestine ISH
apchu745/hu745 + MO1-mllt10 + MO4-tp53 standard conditions Protruding-mouth intestine ISH
apchu745/hu745 + MO2-dot1l + MO4-tp53 standard conditions Protruding-mouth intestine ISH
apchu745/hu745 + MO2-mllt10 + MO4-tp53 standard conditions Protruding-mouth intestine ISH
apchu745/hu745 + MO3-tcf7l2 + MO4-tp53 standard conditions Protruding-mouth intestine ISH
apchu745/hu745 + MO4-tp53 standard conditions Protruding-mouth Not Detected intestine ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-dot1l + MO4-tp53 standard conditions Protruding-mouth intestine well differentiated, abnormal
WT + MO1-mllt10 + MO4-tp53 standard conditions Protruding-mouth intestine well differentiated, abnormal
WT + MO2-dot1l + MO4-tp53 standard conditions Protruding-mouth intestine well differentiated, abnormal
WT + MO2-mllt10 + MO4-tp53 standard conditions Protruding-mouth intestine well differentiated, abnormal
WT + MO3-tcf7l2 + MO4-tp53 standard conditions Protruding-mouth intestine well differentiated, abnormal
WT + MO4-tp53 standard conditions Protruding-mouth intestine well differentiated, abnormal
apchu745/hu745 control Protruding-mouth canonical Wnt signaling pathway decreased process quality, abnormal
Protruding-mouth intestine poorly differentiated, abnormal
apchu745/hu745 + MO1-dot1l + MO4-tp53 standard conditions Protruding-mouth intestine moderately well differentiated, abnormal
apchu745/hu745 + MO1-mllt10 + MO4-tp53 standard conditions Protruding-mouth intestine moderately well differentiated, abnormal
apchu745/hu745 + MO2-dot1l + MO4-tp53 standard conditions Protruding-mouth intestine moderately well differentiated, abnormal
apchu745/hu745 + MO2-mllt10 + MO4-tp53 standard conditions Protruding-mouth intestine moderately well differentiated, abnormal
apchu745/hu745 + MO3-tcf7l2 + MO4-tp53 standard conditions Protruding-mouth intestine well differentiated, abnormal
apchu745/hu745 + MO4-tp53 standard conditions Protruding-mouth canonical Wnt signaling pathway decreased process quality, abnormal
Protruding-mouth intestine poorly differentiated, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Biol.