ZFIN ID: ZDB-FIG-101206-28
Seth et al., 2010 - Core fucosylation is required for midline patterning during zebrafish development. Developmental dynamics : an official publication of the American Association of Anatomists   239(12):3380-3390 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 7 Knockdown of zfApoB phenocopies FucT8 morphants. A,B:islet-1 expression in the optic field is severely reduced (arrowheads) in ApoB morphants, similar to that seen in FucT8 morphants (see Fig. 4A) (27 hpf: 86% of 50 injected embryos showed this phenotype; 48 hpf: 85% of 40 injected embryos showed this phenotype). C: Whole eyes, dissected out from ath-5-labeled embryos, shows a reduced number of ath-5-expressing cells (arrowheads) in 27-hpf ApoB morphants, as seen in FucT8 morphants (see Fig. 4B). Eighty-six percent of 43 injected embryos showed this phenotype. D:pax 2.1 expression in 27-hpf ApoB morphants illustrates a shorter and thicker optic stalk, relative to controls (arrow), and parallels what is seen in FucT8 morphants (see Fig. 4C). Eighty-two percent of 49 injected embryos showed this phenotype. E: Ventral views, anterior to the top. Immunostaining with zn-5 antibody shows loss of RGCs (arrowheads) and optic chiasma (arrow) in 48-hpf ApoB morphant embryos phenocopying that seen in FucT8 morphants (see Fig. 3C). Ninety-eight percent of 47 injected embryos showed this phenotype.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
atoh7 AB control Prim-5 retinal ganglion cell ISH
AB + MO1-apobb.1 standard conditions Prim-5 retinal ganglion cell ISH
isl1 AB control Prim-5 eye ISH
Long-pec eye ISH
AB + MO1-apobb.1 standard conditions Prim-5 Not Detected eye ISH
Long-pec Not Detected eye ISH
pax2a AB control Prim-5 optic stalk ISH
AB + MO1-apobb.1 standard conditions Prim-5 optic stalk ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
zn-5 IHC AB control Long-pec optic chiasm
IHC Long-pec retinal ganglion cell
IHC AB + MO1-apobb.1 standard conditions Long-pec Not Detected optic chiasm
IHC Long-pec Not Detected retinal ganglion cell
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-apobb.1 standard conditions Prim-5 optic stalk decreased length, abnormal
AB + MO1-apobb.1 standard conditions Prim-5 optic stalk increased thickness, abnormal
AB + MO1-apobb.1 standard conditions Prim-5 retinal ganglion cell decreased amount, abnormal
AB + MO1-apobb.1 standard conditions Long-pec retinal ganglion cell decreased amount, abnormal
AB + MO1-apobb.1 standard conditions Long-pec whole organism lacks all parts of type optic chiasm, abnormal
AB + MO2-apobb.1 standard conditions Prim-5 optic stalk decreased length, abnormal
Prim-5 optic stalk increased thickness, abnormal
Prim-5 retinal ganglion cell decreased amount, abnormal
Long-pec retinal ganglion cell decreased amount, abnormal
Long-pec whole organism lacks all parts of type optic chiasm, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.