FIGURE

Fig. S6

ID
ZDB-FIG-101129-21
Publication
Baskin et al., 2010 - Visualizing enveloping layer glycans during zebrafish early embryogenesis
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Fig. S6

Microinjection of Ac4GalNAz and GalNAz afford comparable labeling before 24 hpf. Zebrafish embryos were microinjected with 25 pmol of GalNAz, 25 pmol of Ac4GalNAz, or no sugar, along with the tracer dye rhodamine-dextran, and allowed to develop. At 10 hpf, the embryos were reacted with DIFO-488 (100 μM, 1 h) and then imaged by confocal microscopy. Shown are maximum intensity z-projection fluorescence images and corresponding brightfield images. Green, DIFO-488; red, rhodamine-dextran. Scale bar: 200 μm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA