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Figure Caption
Fig. S6 Microinjection of Ac4GalNAz and GalNAz afford comparable labeling before 24 hpf. Zebrafish embryos were microinjected with 25 pmol of GalNAz, 25 pmol of Ac4GalNAz, or no sugar, along with the tracer dye rhodamine-dextran, and allowed to develop. At 10 hpf, the embryos were reacted with DIFO-488 (100 μM, 1 h) and then imaged by confocal microscopy. Shown are maximum intensity z-projection fluorescence images and corresponding brightfield images. Green, DIFO-488; red, rhodamine-dextran. Scale bar: 200 μm.
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