FIGURE

Fig. 5

ID

ZDB-FIG-101129-16

Publication

Baskin et al., 2010 - Visualizing enveloping layer glycans during zebrafish early embryogenesis

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Fig. 5

Time-lapse monitoring of mitotic cells reveals dramatic glycan reorganization during cell division. (A) Wild-type zebrafish embryos were microinjected with GalNAz, allowed to develop to 10 hpf, reacted with DIFO-488 (100 μM, 1 h), and imaged by confocal microscopy. Arrowheads indicate intense staining at the cleavage furrow of dividing cells. Maximum intensity z-projection images are shown. (B) H2A-GFP transgenic zebrafish embryos were microinjected with GalNAz and allowed to develop to 10 hpf. The embryos were reacted with DIFO-647 (100 μM, 1 h) and imaged by confocal microscopy. Shown are single z-plane frames from a time-lapse movie (S1). Indicated in the top right corner of each image is time (h:min:s). Green, H2A-GFP; red, DIFO-647. (C) H2A-GFP zebrafish were microinjected at the one-cell stage with GalNAz and memCherry mRNA and allowed to develop to 10 hpf. The embryos were reacted with DIFO-647 (100 μM, 1 h) and imaged by confocal microscopy. Blue, H2A-GFP; green, memCherry; red, DIFO-647. Arrowheads indicate location of new membrane between daughter cells. Maximum intensity z-projection images are shown. [Scale bars: 100 μm (A), 20 μm (B, C).]

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA