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ZFIN ID: ZDB-FIG-101011-57
Rai et al., 2010 - DNA demethylase activity maintains intestinal cells in an undifferentiated state following loss of APC. Cell   142(6):930-942 Full text @ Cell
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. S5

Expression of lef1, groucho2, lsd1 and corest in apc Mutant Zebrafish Embryos and their Knockdown with Morpholino, Related to Figure 5

(A) Quantitative RT-PCR showing fold upregulation of lef1, groucho2, lsd1 and corest in apcmcr zebrafish compared apcwt embryos. Transcript levels normalized to 28S rRNA transcripts and then to wild-type values.

(B–F) Bright light images showing gross morphological rescue (B), rescue of yolk structure (C and D), rescue of pectoral fins (marked by arrowhead) (E), and rescue of the somite organization (F) in apcmcr zebrafish injected with lef1 morpholino. Note increased body length of apcmcr zebrafish injected with lef1 Mo compared to uninjected embryos in panel B.

(G) Western blot showing levels of Groucho2 in apcmcr zebrafish embryos (72hpf) injected with control Mo or Groucho2 Mo. Vinculin was used as a loading control.

(H) Western blot showing levels of β-catenin in apcwt and apcmcr zebrafish embryos (72hpf) injected with control Mo or Cox-2 Mo. Actin was used as a loading control.

(I and J) RT-PCR for indicated genes in apcwt and apcmcr embryos injected with control Mo or Cox-2 Mo (I) or treated with DMSO or NS-398 (J). Fold change indicates transcripts levels normalized first to 28S and then to wild-type values, normalized as 1.

(K) RT-PCR results for lef1 in apcmcr embryos injected with wild-type APC (APC SAMP) expressing DNA or a mutant deficient in Axin binding (APC AALP).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
apchu745/hu745 standard conditions Protruding-mouth ball deformed, abnormal
Protruding-mouth extension decreased length, abnormal
Protruding-mouth pectoral fin absent, abnormal
Protruding-mouth somite morphology, abnormal
Protruding-mouth ventral fin fold morphology, abnormal
Protruding-mouth whole organism decreased length, abnormal
apchu745/hu745 + MO2-lef1 standard conditions Protruding-mouth ball obtuse, normal
Protruding-mouth extension morphology, normal
Protruding-mouth pectoral fin present, normal
Protruding-mouth somite morphology, normal
Protruding-mouth whole organism length, normal
Acknowledgments:
ZFIN wishes to thank the journal Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Cell, 142(6), Rai, K., Sarkar, S., Broadbent, T.J., Voas, M., Grossmann, K.F., Nadauld, L.D., Dehghanizadeh, S., Hagos, F.T., Li, Y., Toth, R.K., Chidester, S., Bahr, T.M., Johnson, W.E., Sklow, B., Burt, R., Cairns, B.R., and Jones, D.A., DNA demethylase activity maintains intestinal cells in an undifferentiated state following loss of APC, 930-942, Copyright (2010) with permission from Elsevier. Full text @ Cell