|ZFIN ID: ZDB-FIG-101011-57|
Expression of lef1, groucho2, lsd1 and corest in apc Mutant Zebrafish Embryos and their Knockdown with Morpholino, Related to Figure 5
(A) Quantitative RT-PCR showing fold upregulation of lef1, groucho2, lsd1 and corest in apcmcr zebrafish compared apcwt embryos. Transcript levels normalized to 28S rRNA transcripts and then to wild-type values.
(B–F) Bright light images showing gross morphological rescue (B), rescue of yolk structure (C and D), rescue of pectoral fins (marked by arrowhead) (E), and rescue of the somite organization (F) in apcmcr zebrafish injected with lef1 morpholino. Note increased body length of apcmcr zebrafish injected with lef1 Mo compared to uninjected embryos in panel B.
(G) Western blot showing levels of Groucho2 in apcmcr zebrafish embryos (72hpf) injected with control Mo or Groucho2 Mo. Vinculin was used as a loading control.
(H) Western blot showing levels of β-catenin in apcwt and apcmcr zebrafish embryos (72hpf) injected with control Mo or Cox-2 Mo. Actin was used as a loading control.
(I and J) RT-PCR for indicated genes in apcwt and apcmcr embryos injected with control Mo or Cox-2 Mo (I) or treated with DMSO or NS-398 (J). Fold change indicates transcripts levels normalized first to 28S and then to wild-type values, normalized as 1.
(K) RT-PCR results for lef1 in apcmcr embryos injected with wild-type APC (APC SAMP) expressing DNA or a mutant deficient in Axin binding (APC AALP).
|apchu745/hu745||standard conditions||Protruding-mouth||ball deformed, abnormal|
|Protruding-mouth||extension decreased length, abnormal|
|Protruding-mouth||pectoral fin absent, abnormal|
|Protruding-mouth||somite morphology, abnormal|
|Protruding-mouth||ventral fin fold morphology, abnormal|
|Protruding-mouth||whole organism decreased length, abnormal|
|apchu745/hu745 + MO2-lef1||standard conditions||Protruding-mouth||ball obtuse, normal|
|Protruding-mouth||extension morphology, normal|
|Protruding-mouth||pectoral fin present, normal|
|Protruding-mouth||somite morphology, normal|
|Protruding-mouth||whole organism length, normal|
ZFIN wishes to thank the journal Cell for permission to reproduce figures from this article.
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Reprinted from Cell, 142(6), Rai, K., Sarkar, S., Broadbent, T.J., Voas, M., Grossmann, K.F., Nadauld, L.D., Dehghanizadeh, S., Hagos, F.T., Li, Y., Toth, R.K., Chidester, S., Bahr, T.M., Johnson, W.E., Sklow, B., Burt, R., Cairns, B.R., and Jones, D.A., DNA demethylase activity maintains intestinal cells in an undifferentiated state following loss of APC, 930-942, Copyright (2010) with permission from Elsevier. Full text @ Cell