ZFIN ID: ZDB-FIG-100903-47
Wang et al., 2010 - Moesin1 and Ve-cadherin are required in endothelial cells during in vivo tubulogenesis. Development (Cambridge, England)   137(18):3119-3128 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 7 Knockdown of Moesin1 results in extensive loss of adherens junctions without affecting ZO-1-associated junctions. (A-F) Confocal images of ISVs in the Tg(fli1:egfp)y1 zebrafish embryos that were probed for ZO-1 (red) at 54 hpf. (A-C) ZO-1-associated junctions are seen in a control embryo. (D-F) The ZO-1-associated junctions (arrow) are observed in Moesin1 knockdown embryos but are not well separated from one another. (G-L) Confocal images of ISVs in Tg(fli1:egfp)y1 embryos labeled with Ve-cadherin (red) at 54 hpf. (G-I) Ve-cadherin labeling in a control embryo. (J-L) Moesin1 knockdown causes an extensive loss of Ve-cadherin labeling (arrowheads); some weak Ve-cadherin signals are observed (arrows).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cdh5 Ab1-cdh5 y1Tg control Long-pec blood vessel endothelial cell adherens junction IHC
Ab1-cdh5 y1Tg + MO1-msna standard conditions Long-pec blood vessel endothelial cell adherens junction IHC
EGFP y1Tg control Long-pec intersegmental vessel blood vessel endothelial cell IFL
y1Tg + MO1-msna standard conditions Long-pec intersegmental vessel blood vessel endothelial cell IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tjp1 IHC y1Tg control Long-pec blood vessel endothelial cell bicellular tight junction
IHC y1Tg + MO1-msna standard conditions Long-pec blood vessel endothelial cell bicellular tight junction
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg + MO1-msna standard conditions Long-pec adherens junction organization process quality, abnormal
Long-pec blood vessel endothelial cell adherens junction physical object quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development