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ZIRC
ZFIN ID: ZDB-FIG-100903-48
Wang et al., 2010 - Moesin1 and Ve-cadherin are required in endothelial cells during in vivo tubulogenesis. Development (Cambridge, England)   137(18):3119-3128 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-15 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-15 to Long-pec

Fig. 8 Knockdown of Ve-cadherin impairs lumen formation. (A-F) Confocal images of ISVs in Tg(fli1:egfp)y1 zebrafish embryos probed for Ve-cadherin (red) at 54 hpf. Ve-cadherin labeling (arrows) is observed in control embryos (A-C), but not in Ve-cadherin knockdown embryos (D-F). (G-L) Confocal images of ISVs in Tg(fli1:egfp)y1 embryos labeled with ZO-1 antibody (red) at 54 hpf. ZO-1-associated junctions (arrows) are observed in control embryos (G-I), but are not detected in some regions of the ISV (arrowhead) in a Ve-cadherin knockdown embryo (J-L). (M-P) Confocal images of ISVs in Tg(flk1:moesin1-egfp) embryos at 32 hpf. The primary lumen (arrow) is forming in a control embryo (M). Putative vacuoles or intercellular spaces (arrows in the higher magnification images O and P) are observed throughout the cytoplasm in endothelial cells in Ve-cadherin knockdown embryos (N-P), without formation of primary lumen (N, arrowhead).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cdh5 Ab1-cdh5 y1Tg control Long-pec blood vessel endothelial cell adherens junction IHC
Ab1-cdh5 y1Tg + MO6-cdh5 standard conditions Long-pec Not Detected intersegmental vessel blood vessel endothelial cell IHC
EGFP is1Tg control Prim-15 intersegmental vessel blood vessel endothelial cell IFL
is1Tg + MO6-cdh5 standard conditions Prim-15 intersegmental vessel blood vessel endothelial cell IFL
y1Tg control Long-pec intersegmental vessel blood vessel endothelial cell IFL
y1Tg + MO6-cdh5 standard conditions Long-pec intersegmental vessel blood vessel endothelial cell IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tjp1 IHC y1Tg control Long-pec blood vessel endothelial cell bicellular tight junction
IHC y1Tg + MO6-cdh5 standard conditions Long-pec blood vessel endothelial cell bicellular tight junction
Phenotype Details
Fish Conditions Stage Phenotype
is1Tg + MO6-cdh5 standard conditions Prim-15 blood vessel lumenization process quality, abnormal
Prim-15 intersegmental vessel unlumenized, abnormal
y1Tg + MO6-cdh5 standard conditions Long-pec blood vessel lumenization process quality, abnormal
Long-pec intersegmental vessel unlumenized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development