PUBLICATION

Moesin1 and Ve-cadherin are required in endothelial cells during in vivo tubulogenesis

Authors
Wang, Y., Kaiser, M.S., Larson, J.D., Nasevicius, A., Clark, K.J., Wadman, S.A., Roberg-Perez, S.E., Ekker, S.C., Hackett, P.B., McGrail, M., and Essner, J.J.
ID
ZDB-PUB-100826-21
Date
2010
Source
Development (Cambridge, England)   137(18): 3119-3128 (Journal)
Registered Authors
Clark, Karl, Ekker, Stephen C., Essner, Jeffrey, Hackett, Perry B., Larson, Jon D., McGrail, Maura, Nasevicius, Aidas, Wang, Guixue
Keywords
Zebrafish, Endothelial tubulogenesis, Angiogenesis, Lumen, Vacuole, Actin, Moesin1 (Moesin a), Ve-cadherin
MeSH Terms
  • Adherens Junctions/metabolism
  • Animals
  • Antigens, CD/genetics
  • Antigens, CD/metabolism*
  • Cadherins/genetics
  • Cadherins/metabolism*
  • Cell Polarity
  • Embryo, Nonmammalian/blood supply*
  • Embryo, Nonmammalian/metabolism*
  • Endothelial Cells/cytology
  • Endothelial Cells/metabolism*
  • Gene Expression Regulation, Developmental
  • Membrane Proteins/genetics
  • Membrane Proteins/metabolism
  • Microfilament Proteins/deficiency
  • Microfilament Proteins/genetics
  • Microfilament Proteins/metabolism*
  • Neovascularization, Physiologic
  • Phosphoproteins/genetics
  • Phosphoproteins/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zonula Occludens-1 Protein
PubMed
20736288 Full text @ Development
Abstract
Endothelial tubulogenesis is a crucial step in the formation of functional blood vessels during angiogenesis and vasculogenesis. Here, we use in vivo imaging of living zebrafish embryos expressing fluorescent fusion proteins of beta-Actin, alpha-Catenin, and the ERM family member Moesin1 (Moesin a), to define a novel cord hollowing process that occurs during the initial stages of tubulogenesis in intersegmental vessels (ISVs) in the embryo. We show that the primary lumen elongates along cell junctions between at least two endothelial cells during embryonic angiogenesis. Moesin1-EGFP is enriched around structures that resemble intracellular vacuoles, which fuse with the luminal membrane during expansion of the primary lumen. Analysis of silent heart mutant embryos shows that initial lumen formation in the ISVs is not dependent on blood flow; however, stabilization of a newly formed lumen is dependent upon blood flow. Zebrafish moesin1 knockdown and cell transplantation experiments demonstrate that Moesin1 is required in the endothelial cells of the ISVs for in vivo lumen formation. Our analyses suggest that Moesin1 contributes to the maintenance of apical/basal cell polarity of the ISVs as defined by adherens junctions. Knockdown of the adherens junction protein Ve-cadherin disrupts formation of the apical membrane and lumen in a cell-autonomous manner. We suggest that Ve-cadherin and Moesin1 function to establish and maintain apical/basal polarity during multicellular lumen formation in the ISVs.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes