ZFIN ID: ZDB-FIG-100903-21
Dutta et al., 2010 - Kctd15 inhibits neural crest formation by attenuating Wnt/β-catenin signaling output. Development (Cambridge, England)   137(18):3013-3018 Full text @ Development
Anatomical Terms:
Stage Range: 1-4 somites to Prim-5
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Fig. 1 Zebrafish kctd15 expression pattern, loss-of-function and gain-of-function phenotypes. (A-E) Wild-type embryos were hybridized in situ, with probes indicated at lower right, stages shown at lower left. Dorsal views, anterior towards the top (A-D) or left (E). (A,B) Cells in the neural plate border (NPB) express kctd15a and kctd15b. (C) dlx3b (red) and kctd15a (blue) are coexpressed at the NPB. (D,D inset) kctd15a (blue) is excluded from the premigratory neural crest (NC) expressing foxd3 (red). (E) kctd15a expression at 24 hpf. arrows, hindbrain neurons. (F-M) Embryos were injected with RNA (F-I) or MO (J-M). Lateral views, anterior to the left. Overexpression of kctd15a (G, 75/100) and ΔCkctd15a (I, 82/100) results in loss of pigmentation and a short tail, whereas ΔNkctd15a mRNA-injected embryos were normal (H, 60/60). Co-injection of Kctd15a/15b MO results in a small head and abnormal somites (K, 85/100) and subsequently increased dorsal pigmentation (M, 91/100) and loss of jaw elements (M inset, arrowhead). s, somite; t, telencephalon; teg, tegmentum; tg, trigeminal placode; LLP, lateral line primordia. Scale bars: 50 μm in A-D,L,M inset; 80 μm in E; 100 μm in F-M.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlx3b WT standard conditions 1-4 somites neural plate ISH
foxd3 WT standard conditions 1-4 somites neural crest cell ISH
kctd15a WT standard conditions 1-4 somites neural plate ISH
Prim-5 dorsolateral placode ISH
Prim-5 hindbrain CNS neuron (sensu Vertebrata) ISH
Prim-5 lateral line primordium ISH
Prim-5 tegmentum ISH
Prim-5 telencephalon ISH
kctd15b WT standard conditions 1-4 somites neural plate ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-kctd15a + MO1-kctd15b standard conditions Prim-5 cell death increased occurrence, abnormal
Prim-5 head decreased size, abnormal
Prim-5 somite morphology, abnormal
Protruding-mouth mandibular arch skeleton aplastic, abnormal
Protruding-mouth pharyngeal arch morphology, abnormal
Protruding-mouth pigmentation increased occurrence, abnormal
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development