ZFIN ID: ZDB-FIG-100903-23 |
Gene: | |
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Fish: | |
Knockdown Reagents: | |
Anatomical Terms: | |
Stage: | 1-4 somites |
Fish: | |
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Knockdown Reagent: | |
Observed In: | |
Stage: | 1-4 somites |
Fig. 3 Kctd15 inhibits the output of canonical Wnt signaling. (A) foxd3 expression in control embryos. (B) Loss of foxd3 expression in wnt8.1 morphants. (C) Injection of Wnt8.1 MO together with Kctd15a/15b MOs rescued foxd3 expression. (D) Quantification of A-C; number of embryos (n) is given above bars. (E-J) foxd3 expression in embryos treated with LiCl (E-G). Arrow in G indicates expanded expression in 26/30 embryos. (H-J) LiCl did not rescue foxd3 expression in kctd15a mRNA-injected embryos (I, 40/40; J, 50/50; numbers indicate embryos with appearance as shown in the figure). (K-M) Inhibition of foxd3 by kctd15a (K) was rescued by β-cat* mRNA (M, 41/50 showing foxd3 expression in NC domain). β-cat* mRNA alone induced ectopic expression of foxd3 (L, arrow, 25/31). (A-C,E-M) 1-somite stage; dorsal views, anterior towards top. Scale bar: 100 μm. |
Gene | Antibody | Fish | Conditions | Stage | Qualifier | Anatomy | Assay |
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foxd3 | WT | control | 1-4 somites | neural crest cell | ISH | ||
1-4 somites | notochord | ISH | |||||
WT + MO1-kctd15a + MO1-kctd15b + MO1-wnt8a | standard conditions | 1-4 somites | neural crest cell | ISH | |||
1-4 somites | notochord | ISH | |||||
WT + MO1-wnt8a | standard conditions | 1-4 somites | Not Detected | neural crest cell | ISH | ||
1-4 somites | Not Detected | notochord | ISH |
Fish | Conditions | Stage | Phenotype |
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WT + MO1-wnt8a | standard conditions | 1-4 somites | neural crest cell morphology, abnormal |
1-4 somites | neural crest cell differentiation disrupted, abnormal |
Acknowledgments: | ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development |
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