| ZFIN ID: ZDB-FIG-100903-23 |
| Gene: | |
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| Fish: | |
| Knockdown Reagents: | |
| Anatomical Terms: | |
| Stage: | 1-4 somites |
| Fish: | |
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| Knockdown Reagent: | |
| Observed In: | |
| Stage: | 1-4 somites |
Fig. 3 Kctd15 inhibits the output of canonical Wnt signaling. (A) foxd3 expression in control embryos. (B) Loss of foxd3 expression in wnt8.1 morphants. (C) Injection of Wnt8.1 MO together with Kctd15a/15b MOs rescued foxd3 expression. (D) Quantification of A-C; number of embryos (n) is given above bars. (E-J) foxd3 expression in embryos treated with LiCl (E-G). Arrow in G indicates expanded expression in 26/30 embryos. (H-J) LiCl did not rescue foxd3 expression in kctd15a mRNA-injected embryos (I, 40/40; J, 50/50; numbers indicate embryos with appearance as shown in the figure). (K-M) Inhibition of foxd3 by kctd15a (K) was rescued by β-cat* mRNA (M, 41/50 showing foxd3 expression in NC domain). β-cat* mRNA alone induced ectopic expression of foxd3 (L, arrow, 25/31). (A-C,E-M) 1-somite stage; dorsal views, anterior towards top. Scale bar: 100 μm. |
| Gene | Antibody | Fish | Conditions | Stage | Qualifier | Anatomy | Assay |
|---|---|---|---|---|---|---|---|
| foxd3 | WT | control | 1-4 somites | neural crest cell | ISH | ||
| 1-4 somites | notochord | ISH | |||||
| WT + MO1-kctd15a + MO1-kctd15b + MO1-wnt8a | standard conditions | 1-4 somites | neural crest cell | ISH | |||
| 1-4 somites | notochord | ISH | |||||
| WT + MO1-wnt8a | standard conditions | 1-4 somites | Not Detected | neural crest cell | ISH | ||
| 1-4 somites | Not Detected | notochord | ISH |
| Fish | Conditions | Stage | Phenotype |
|---|---|---|---|
| WT + MO1-wnt8a | standard conditions | 1-4 somites | neural crest cell differentiation disrupted, abnormal |
| 1-4 somites | neural crest cell morphology, abnormal |
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