ZFIN ID: ZDB-FIG-100903-23
Dutta et al., 2010 - Kctd15 inhibits neural crest formation by attenuating Wnt/β-catenin signaling output. Development (Cambridge, England)   137(18):3013-3018 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 1-4 somites
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: 1-4 somites

Fig. 3 Kctd15 inhibits the output of canonical Wnt signaling. (A) foxd3 expression in control embryos. (B) Loss of foxd3 expression in wnt8.1 morphants. (C) Injection of Wnt8.1 MO together with Kctd15a/15b MOs rescued foxd3 expression. (D) Quantification of A-C; number of embryos (n) is given above bars. (E-J) foxd3 expression in embryos treated with LiCl (E-G). Arrow in G indicates expanded expression in 26/30 embryos. (H-J) LiCl did not rescue foxd3 expression in kctd15a mRNA-injected embryos (I, 40/40; J, 50/50; numbers indicate embryos with appearance as shown in the figure). (K-M) Inhibition of foxd3 by kctd15a (K) was rescued by β-cat* mRNA (M, 41/50 showing foxd3 expression in NC domain). β-cat* mRNA alone induced ectopic expression of foxd3 (L, arrow, 25/31). (A-C,E-M) 1-somite stage; dorsal views, anterior towards top. Scale bar: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
foxd3 WT control 1-4 somites neural crest cell ISH
1-4 somites notochord ISH
WT + MO1-kctd15a + MO1-kctd15b + MO1-wnt8a standard conditions 1-4 somites neural crest cell ISH
1-4 somites notochord ISH
WT + MO1-wnt8a standard conditions 1-4 somites Not Detected neural crest cell ISH
1-4 somites Not Detected notochord ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-wnt8a standard conditions 1-4 somites neural crest cell differentiation disrupted, abnormal
1-4 somites neural crest cell morphology, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development