ZFIN ID: ZDB-FIG-100903-23
Dutta et al., 2010 - Kctd15 inhibits neural crest formation by attenuating Wnt/β-catenin signaling output. Development (Cambridge, England)   137(18):3013-3018 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 1-4 somites
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: 1-4 somites

Fig. 3 Kctd15 inhibits the output of canonical Wnt signaling. (A) foxd3 expression in control embryos. (B) Loss of foxd3 expression in wnt8.1 morphants. (C) Injection of Wnt8.1 MO together with Kctd15a/15b MOs rescued foxd3 expression. (D) Quantification of A-C; number of embryos (n) is given above bars. (E-J) foxd3 expression in embryos treated with LiCl (E-G). Arrow in G indicates expanded expression in 26/30 embryos. (H-J) LiCl did not rescue foxd3 expression in kctd15a mRNA-injected embryos (I, 40/40; J, 50/50; numbers indicate embryos with appearance as shown in the figure). (K-M) Inhibition of foxd3 by kctd15a (K) was rescued by β-cat* mRNA (M, 41/50 showing foxd3 expression in NC domain). β-cat* mRNA alone induced ectopic expression of foxd3 (L, arrow, 25/31). (A-C,E-M) 1-somite stage; dorsal views, anterior towards top. Scale bar: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
foxd3 WT control 1-4 somites neural crest cell ISH
1-4 somites notochord ISH
WT + MO1-kctd15a + MO1-kctd15b + MO1-wnt8a standard conditions 1-4 somites neural crest cell ISH
1-4 somites notochord ISH
WT + MO1-wnt8a standard conditions 1-4 somites Not Detected neural crest cell ISH
1-4 somites Not Detected notochord ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-wnt8a standard conditions 1-4 somites neural crest cell morphology, abnormal
1-4 somites neural crest cell differentiation disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development