ZFIN ID: ZDB-FIG-100806-61
Yang et al., 2010 - Regulatory interactions specifying Kolmer-Agduhr interneurons. Development (Cambridge, England)   137(16):2713-2722 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Prim-25

Fig. 6 Mapping co-expression of marker genes and GABA in the zebrafish lateral floor plate. (A-C) nkx2.9 (A) and tal2 (B) mRNA expression and a merged view (C). nkx2.9-positive cells (arrowheads) co-express tal2. (D-F) GABA immunohistochemistry (D), tal2 in situ hybridization (E) and a merged view (F). tal2-expressing cells (arrowheads) are GABAergic. (G-I) gata2 (G) and tal2 (H) mRNA expression and a merged view (I). gata2 and tal2 mRNAs (arrowheads) are co-expressed in the lateral floor plate. More dorsally in the spinal cord, not all gata2-positive cells are tal2-positive. (J-L) gata3 (J) and tal2 (K) mRNA expression and merge (L). gata3 mRNA-expressing cells co-express tal2 mRNA in the lateral floor plate. However, more dorsally, only a proportion of gata3-positive cells also expresses tal2 mRNA. The tal2-negative, gata2- and gata3-positive cells are probably V2b/VeLD interneurons (Batista et al., 2008). (M-O) sim1 (M) and tal2 (N) mRNA expression and merge (O). sim1-expressing interneurons and tal2-expressing cells in the lateral floor plate are distinct in most cases. Only in 25% of cells did we find co-expression of the two markers. (A-F) Projections of several sections. (G-O) Single confocal planes. Embryos were 24 (A-L) or 36 (M-O) hpf. Dorsal up, anterior left. Scale bar: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gata2a WT standard conditions Prim-5 lateral floor plate GABAergic neuron ISH
Prim-5 lateral floor plate Kolmer-Agduhr neuron ISH
Prim-5 spinal cord Kolmer-Agduhr neuron ISH
Prim-5 VeLD ISH
gata3 WT standard conditions Prim-5 lateral floor plate GABAergic neuron ISH
nkx2.9 WT standard conditions Prim-5 lateral floor plate GABAergic neuron ISH
sim1a WT standard conditions Prim-25 lateral floor plate interneuron ISH
tal2 WT standard conditions Prim-5 lateral floor plate GABAergic neuron ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development