ZFIN ID: ZDB-FIG-100806-59
Yang et al., 2010 - Regulatory interactions specifying Kolmer-Agduhr interneurons. Development (Cambridge, England)   137(16):2713-2722 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 3 tal2 expression in the lateral floor plate depends on nkx2.2a, nkx2.2b and nkx2.9. (A,B) Control (A) and Mo-nkx2.9-injected (B) zebrafish embryo. Knockdown of nkx2.9 leads to a reduction of tal2-positive cells in the lateral floor plate (arrow) but dorsal tal2-positive cells are unaffected (arrowhead). (C,D) Control (C) and Mo-nkx2.9/Mo-nkx2.2a-injected (D) embryos. There is a reduction of tal2-positive cells in the lateral floor plate, whereas tal2-expressing cells (arrowhead) further dorsal in the spinal cord are unaffected in the double-knockdown embryos. (E,F) Control (E) and Mo-nkx2.9/Mo-nkx2.2b-injected (F) embryos. There is a reduction of tal2-positive cells in the lateral floor plate (arrow) in the double-knockdown embryos. (G,H) Control (G) and Mo-nkx2.2a/Mo-nkx2.2b-injected (H) embryos. Double knockdown of nkx2.2a and nkx2.2b caused little reduction of tal2-positive cells (arrow) in the lateral floor plate. (I,J) Control (I) and triple-knockdown (J) embryos. Knockdown of all three Nkx2 genes abolished tal2-positive cells in the lateral floor plate (arrow). tal2-expressing cells (arrowhead) further dorsal in the spinal cord were unaffected in these knockdown embryos. (K) Cross-section through the trunk at hindgut extension of a triple-knockdown embryo. tal2-expressing cells in the lateral floor plate (arrow) are missing, whereas more dorsally located tal2-positive cells are present (arrowhead). Note that controls represent injections of mismatch morpholinos or combinations thereof (Table 1). Representative lateral views of the spinal cord over the hindgut extension are shown. Embryos (24 hpf) are shown with anterior left, dorsal up. Scale bar: 25 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
tal2 WT control Prim-5 lateral floor plate ISH
Prim-5 spinal cord ISH
Prim-5 spinal cord interneuron ISH
WT + MO1-nkx2.9 standard conditions Prim-5 lateral floor plate ISH
Prim-5 spinal cord ISH
Prim-5 spinal cord interneuron ISH
WT + MO1-nkx2.9 + MO2-nkx2.2b standard conditions Prim-5 lateral floor plate ISH
Prim-5 spinal cord ISH
Prim-5 spinal cord interneuron ISH
WT + MO1-nkx2.9 + MO2-nkx2.2b + MO4-nkx2.2a standard conditions Prim-5 Not Detected lateral floor plate ISH
Prim-5 spinal cord ISH
Prim-5 spinal cord interneuron ISH
WT + MO1-nkx2.9 + MO4-nkx2.2a standard conditions Prim-5 lateral floor plate ISH
Prim-5 spinal cord ISH
Prim-5 spinal cord interneuron ISH
WT + MO2-nkx2.2b + MO4-nkx2.2a standard conditions Prim-5 lateral floor plate ISH
Prim-5 spinal cord ISH
Prim-5 spinal cord interneuron ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-nkx2.9 standard conditions Prim-5 spinal cord interneuron cellular quality, abnormal
Prim-5 ventral spinal cord interneuron differentiation disrupted, abnormal
WT + MO1-nkx2.9 + MO2-nkx2.2b standard conditions Prim-5 spinal cord interneuron cellular quality, abnormal
Prim-5 ventral spinal cord interneuron differentiation disrupted, abnormal
WT + MO1-nkx2.9 + MO2-nkx2.2b + MO4-nkx2.2a standard conditions Prim-5 spinal cord interneuron cellular quality, abnormal
Prim-5 ventral spinal cord interneuron differentiation disrupted, abnormal
WT + MO1-nkx2.9 + MO4-nkx2.2a standard conditions Prim-5 spinal cord interneuron cellular quality, abnormal
Prim-5 ventral spinal cord interneuron differentiation disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development