ZFIN ID: ZDB-FIG-090401-49
Toro et al., 2009 - Identification of differentially expressed genes in the zebrafish hypothalamic-pituitary axis. Gene Expression Patterns   9(4):200-208 Full text @ Gene Expr. Patterns
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes: kctd4, nms, oacyl, palmdb, pcsk1nl (all 8) expand
Fish: WT
Anatomical Terms: hypophysis, hypothalamus, telencephalon
Stage: Adult

Fig. 4 Genes expressed in adult hypothalamic–pituitary axis. (A–H) Genes isolated in the hypothalamic library expressed in the adult. (A–D) kctd4 and psd3l were expressed in adult telencephalon and hypothalamus corresponding to the expression domains we found in 1–5 day old larvae (Fig. 2E–H and P–S). (E and F) In contrast, nppcl was not expressed in the hypothalamus between 1 d and 5 d; however, we found expression in a distinct set of cells in the adult hypothalamus. (G and H) The novel gene uo:ion006 was not expressed in embryonic and larval tissues at all, however, we detected hypothalamic expression in the adult. (I–P) We made similar observations for the pituitary library: (I–L) genes such as uo:ion002 and uo:ion003 that were expressed in the embryonic larval HP were also found in the adult pituitary (I and J) or hypothalamus and pituitary (K and L). (M–P) pncr2 that was not detected in embryonic and larval HP, or nms that was not detected in embryos and larvae at all, were both detected in adult HP (M and N) and adult pituitary (O and P). (A–P) 15 μm, sagittal sections of the adult brain, anterior left, dorsal up. (A, C, E, G, I, K, M, O) scale bar 1 mm; (B, D, F, H, J, L, N, P) scale bar 500 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
kctd4 WT standard conditions Adult hypothalamus ISH
Adult telencephalon ISH
nms WT standard conditions Adult hypophysis ISH
oacyl WT standard conditions Adult hypophysis ISH
palmdb WT standard conditions Adult hypothalamus ISH
pcsk1nl WT standard conditions Adult hypophysis ISH
Adult hypothalamus ISH
pnrc2 WT standard conditions Adult hypothalamus ISH
psd3l WT standard conditions Adult hypothalamus ISH
Adult telencephalon ISH
wu:fj39g12 WT standard conditions Adult hypothalamus ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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Reprinted from Gene Expression Patterns, 9(4), Toro, S., Wegner, J., Muller, M., Westerfield, M., and Varga, Z.M., Identification of differentially expressed genes in the zebrafish hypothalamic-pituitary axis, 200-208, Copyright (2009) with permission from Elsevier. Full text @ Gene Expr. Patterns