ZFIN ID: ZDB-FIG-090401-47
Toro et al., 2009 - Identification of differentially expressed genes in the zebrafish hypothalamic-pituitary axis. Gene Expression Patterns   9(4):200-208 Full text @ Gene Expr. Patterns
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes: adcyap1b, camk1db, kctd4, psd3l, serpini1 (all 6) expand
Fish: WT
Anatomical Terms: cerebellum, hindbrain, hypothalamus, midbrain, neural crest (all 7) expand
Stage Range: Prim-5 to Protruding-mouth

Fig. 2 Genes expressed in the hypothalamus. (A–D) adcyap1b and kctd4 (E–H) are expressed in the hypothalamus. adcyap1b is expressed in the telencephalon from 24 to 72 h (A–C) and in the hypothalamus at 48 and 72 h (B–C). (D) adcyap1b and kctd4 (H) are expressed by hypothalamic cells (black) close to the adenohypophysis (demarcated by pomc, red) but not in neurohypophysial cells. (I–K) camk1d is expressed in the dorsal hypothalamus, telencephalon, and midbrain at 48 and 72 h. (L–N) stmn1b is expressed in telencephalic, mesencephalic, and hypothalamic cells between 24 and 72 h. (O) The section at 72 h suggests that ventral hypothalamic cells and some, but not all neurohypophysial cells express the gene. (P–R) psd3l is also expressed in telencephalon and hypothalamus between 24 and 72 h. (S) Hypothalamic cells express psd3l. (T–V) serpini1 is expressed in the dorsal hypothalamus at 24 h (T), at 48 h in telencephalon, diencephalon, and mesencephalon. (W) serpini1 is expressed by hypothalamic cells (blue) close to the adenohypophysis (demarcated by pomc, red) but not in neurohypophysial cells. Side views, anterior to the left. (A, E, L, P, T) 24 h; (B, F, I, M, Q, U) 48 h; (C, G, J, N, R, V) 72 h; Scale bar 100 μm. (D, H, K, O, S, W) 15 μm sagittal sections through the pituitary and adjacent hypothalamus at 72 h, scale bar 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
adcyap1b WT standard conditions Prim-5 telencephalon ISH
Long-pec cerebellum ISH
Long-pec hypothalamus ISH
Long-pec midbrain ISH
Long-pec telencephalon ISH
Protruding-mouth cerebellum ISH
Protruding-mouth hypothalamus ISH
Protruding-mouth midbrain ISH
Protruding-mouth telencephalon ISH
camk1db WT standard conditions Long-pec hypothalamus ISH
Long-pec midbrain ISH
Long-pec telencephalon ISH
Protruding-mouth hypothalamus ISH
Protruding-mouth midbrain ISH
Protruding-mouth telencephalon ISH
kctd4 WT standard conditions Prim-5 hypothalamus ISH
Prim-5 telencephalon ISH
Long-pec hindbrain ISH
Long-pec hypothalamus ISH
Long-pec telencephalon ISH
Protruding-mouth hypothalamus ISH
Protruding-mouth telencephalon ISH
psd3l WT standard conditions Prim-5 hypothalamus ISH
Prim-5 telencephalon ISH
Long-pec hypothalamus ISH
Long-pec telencephalon ISH
Protruding-mouth hypothalamus ISH
Protruding-mouth telencephalon ISH
serpini1 WT standard conditions Prim-5 hypothalamus ISH
Long-pec hypothalamus ISH
Long-pec midbrain ISH
Long-pec telencephalon ISH
Protruding-mouth hypothalamus ISH
Protruding-mouth midbrain ISH
Protruding-mouth telencephalon ISH
stmn1b WT standard conditions Prim-5 hypothalamus ISH
Prim-5 neural crest ISH
Prim-5 telencephalon ISH
Long-pec hypothalamus ISH
Long-pec midbrain ISH
Long-pec telencephalon ISH
Protruding-mouth hypothalamus ISH
Protruding-mouth midbrain ISH
Protruding-mouth neurohypophysis ISH
Protruding-mouth telencephalon ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Gene Expression Patterns for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Gene Expression Patterns, 9(4), Toro, S., Wegner, J., Muller, M., Westerfield, M., and Varga, Z.M., Identification of differentially expressed genes in the zebrafish hypothalamic-pituitary axis, 200-208, Copyright (2009) with permission from Elsevier. Full text @ Gene Expr. Patterns