ZFIN ID: ZDB-FIG-081125-4
Morton et al., 2008 - microRNA-138 modulates cardiac patterning during embryonic development. Proceedings of the National Academy of Sciences of the United States of America   105(46):17830-17835 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 2 miR-138 knockdown leads to expansion of AVC-specific gene expression into the ventricle. (A–H) Ventral views of 48-hpf embryos after mRNA in situ hybridization focusing on head (h) and heart (dotted lines) regions. Atrial (A and B) and ventricular (C and D) markers were similar in wild-type (wt) (A and C) and miR-138mo-1 embryos (B and D). Expression of AVC-specific markers cspg2 (E and F) and notch1b (G and H) expanded into the ventricles (v) in miR-138mo-1 embryos (F and H) but not in wild-type embryos (E and G). (a, atrium; amhc, atrial myosin heavy chain; vmhc, ventricular myosin heavy chain.)

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myh6 AB standard conditions Long-pec atrium ISH
AB + MO1-mir138 standard conditions Long-pec atrium ISH
myh7 AB standard conditions Long-pec cardiac ventricle ISH
AB + MO1-mir138 standard conditions Long-pec cardiac ventricle ISH
notch1b AB standard conditions Long-pec atrioventricular canal ISH
AB + MO1-mir138 standard conditions Long-pec atrioventricular canal ISH
Long-pec cardiac ventricle ISH
vcana AB standard conditions Long-pec atrioventricular canal ISH
AB + MO1-mir138 standard conditions Long-pec atrioventricular canal ISH
Long-pec cardiac ventricle ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-mir138 standard conditions Long-pec cardiac ventricle development disrupted, abnormal
Acknowledgments:
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Open Access.
Full text @ Proc. Natl. Acad. Sci. USA