| ZFIN ID: ZDB-FIG-081125-4 |
Morton et al., 2008
- microRNA-138 modulates cardiac patterning during embryonic development.
Proceedings of the National Academy of Sciences of the United States of America
105(46):17830-17835
Full text @ Proc. Natl. Acad. Sci. USA
EXPRESSION / LABELING:
| Genes: | |
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| Fish: | |
| Knockdown Reagent: | |
| Anatomical Terms: | |
| Stage: | Long-pec |
PHENOTYPE:
| Fish: | |
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| Knockdown Reagent: | |
| Observed In: | |
| Stage: | Long-pec |
Fig. 2 miR-138 knockdown leads to expansion of AVC-specific gene expression into the ventricle. (A–H) Ventral views of 48-hpf embryos after mRNA in situ hybridization focusing on head (h) and heart (dotted lines) regions. Atrial (A and B) and ventricular (C and D) markers were similar in wild-type (wt) (A and C) and miR-138mo-1 embryos (B and D). Expression of AVC-specific markers cspg2 (E and F) and notch1b (G and H) expanded into the ventricles (v) in miR-138mo-1 embryos (F and H) but not in wild-type embryos (E and G). (a, atrium; amhc, atrial myosin heavy chain; vmhc, ventricular myosin heavy chain.) |
Gene Expression Details
| Gene | Antibody | Fish | Conditions | Stage | Anatomy | Assay |
|---|---|---|---|---|---|---|
| myh6 | AB | standard conditions | Long-pec | atrium | ISH | |
| AB + MO1-mir138 | standard conditions | Long-pec | atrium | ISH | ||
| myh7 | AB | standard conditions | Long-pec | cardiac ventricle | ISH | |
| AB + MO1-mir138 | standard conditions | Long-pec | cardiac ventricle | ISH | ||
| notch1b | AB | standard conditions | Long-pec | atrioventricular canal | ISH | |
| AB + MO1-mir138 | standard conditions | Long-pec | atrioventricular canal | ISH | ||
| Long-pec | cardiac ventricle | ISH | ||||
| vcana | AB | standard conditions | Long-pec | atrioventricular canal | ISH | |
| AB + MO1-mir138 | standard conditions | Long-pec | atrioventricular canal | ISH | ||
| Long-pec | cardiac ventricle | ISH |
Antibody Labeling Details
No data available
Phenotype Details
| Fish | Conditions | Stage | Phenotype |
|---|---|---|---|
| AB + MO1-mir138 | standard conditions | Long-pec | cardiac ventricle development disrupted, abnormal |
| Acknowledgments: |
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Open Access.
Full text @ Proc. Natl. Acad. Sci. USA
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