ZFIN ID: ZDB-FIG-081022-7
Krens et al., 2008 - ERK1 and ERK2 MAPK are key regulators of distinct gene sets in zebrafish embryogenesis. BMC Genomics   9:196 Full text @ BMC Genomics
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: 30%-epiboly

Fig. 4 Quantitative real-time PCR confirmation of the microarray results. (A-G) qPCR was performed on seven genes that showed differential regulation of expression in response to knockdown of either ERK1, ERK2 and the standard control MO control:cdh2 (NM_131081), mycn (NM_212614), erm (NM_131205), cfos (NM_205569), mos (NM_205580), snai1a (NM_131066) and vegf (NM_131408), correlated to the β-actin housekeeping gene. A comparison of the fold changes in expression of these genes, detected by qPRC assay and microarray, are listed in a table (H). ⇑ = induction of expression, ⇓ = repression of expression, compared to the standard control MO (A-C, red line).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cdh2 WT control 30%-epiboly whole organism RTPCR
WT + MO1-mapk1 standard conditions 30%-epiboly whole organism RTPCR
WT + MO3-mapk3 standard conditions 30%-epiboly whole organism RTPCR
etv5b WT control 30%-epiboly whole organism RTPCR
WT + MO1-mapk1 standard conditions 30%-epiboly whole organism RTPCR
WT + MO3-mapk3 standard conditions 30%-epiboly whole organism RTPCR
fosab WT control 30%-epiboly whole organism RTPCR
WT + MO1-mapk1 standard conditions 30%-epiboly whole organism RTPCR
WT + MO3-mapk3 standard conditions 30%-epiboly whole organism RTPCR
mos WT control 30%-epiboly whole organism RTPCR
WT + MO1-mapk1 standard conditions 30%-epiboly whole organism RTPCR
WT + MO3-mapk3 standard conditions 30%-epiboly whole organism RTPCR
mycn WT control 30%-epiboly whole organism RTPCR
WT + MO1-mapk1 standard conditions 30%-epiboly whole organism RTPCR
WT + MO3-mapk3 standard conditions 30%-epiboly whole organism RTPCR
snai1a WT control 30%-epiboly whole organism RTPCR
WT + MO1-mapk1 standard conditions 30%-epiboly whole organism RTPCR
WT + MO3-mapk3 standard conditions 30%-epiboly whole organism RTPCR
vegfaa WT control 30%-epiboly whole organism RTPCR
WT + MO1-mapk1 standard conditions 30%-epiboly whole organism RTPCR
WT + MO3-mapk3 standard conditions 30%-epiboly whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Genomics