ZFIN ID: ZDB-FIG-070802-23
Plaster et al., 2007 - REREa/Atrophin-2 interacts with histone deacetylase and Fgf8 signaling to regulate multiple processes of zebrafish development. Developmental dynamics : an official publication of the American Association of Anatomists   236(7):1891-1904 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 1-4 somites to 14-19 somites
PHENOTYPE:
Fish:
Observed In:
Stage Range: 1-4 somites to Prim-5

Fig. 5 rerea interacts genetically with fgf8 in posterior mesoderm development. A-C: Lateral views of live 24 hpf embryos with genotypes in the upper right corner. A-C′: Inset panels show the corresponding lateral views of diphosphorylated ERK1&2 (pERK) immunostained tail buds at 13 somites. Arrows denote the position of pERK staining. D-I: Whole-mount in situ hybridizations of papc at 4 somites (D-F) or myod with Ntl immunostains at 16 somites (G-I) shown in dorsal view with genotypes shown in the upper right corner. The arrowhead in I marks a gap in Ntl staining. "WT(bab)" notes that there is no observable difference between wild-type (WT) and bab embryos. ace shows a slight reduction in the amount of posterior mesoderm (B) and presomitic mesoderm as seen by papc expression at four somites (E), while bab;ace (C,F) shows a severe reduction in comparison to wild-type or bab (A,D).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myod1 WT standard conditions 14-19 somites paraxial mesoderm ISH
fgf8ati282a/ti282a standard conditions 14-19 somites paraxial mesoderm ISH
fgf8ati282a/ti282a; rereatb210/tb210 standard conditions 14-19 somites paraxial mesoderm ISH
rereatb210/tb210 standard conditions 14-19 somites paraxial mesoderm ISH
pcdh8 WT standard conditions 1-4 somites segmental plate ISH
fgf8ati282a/ti282a standard conditions 1-4 somites segmental plate ISH
fgf8ati282a/ti282a; rereatb210/tb210 standard conditions 1-4 somites segmental plate ISH
rereatb210/tb210 standard conditions 1-4 somites segmental plate ISH
tbxta WT standard conditions 14-19 somites axial mesoderm ISH
fgf8ati282a/ti282a standard conditions 14-19 somites axial mesoderm ISH
fgf8ati282a/ti282a; rereatb210/tb210 standard conditions 14-19 somites axial mesoderm ISH
rereatb210/tb210 standard conditions 14-19 somites axial mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
fgf8ati282a/ti282a standard conditions Prim-5 post-vent region mesoderm morphology, normal
Prim-5 post-vent region morphology, normal
fgf8ati282a/ti282a; rereatb210/tb210 standard conditions 1-4 somites segmental plate decreased size, abnormal
14-19 somites post-vent region mesoderm decreased amount, abnormal
Prim-5 post-vent region mesoderm aplastic, abnormal
Prim-5 post-vent region truncated, abnormal
rereatb210/tb210 standard conditions Prim-5 post-vent region mesoderm morphology, normal
Prim-5 post-vent region morphology, normal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.