ZFIN ID: ZDB-FIG-070502-24
Zhang et al., 2007 - The characterization of zebrafish antimorphic mib alleles reveals that Mib and Mind bomb-2 (Mib2) function redundantly. Developmental Biology   305(1):14-27 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 26+ somites to High-pec

Fig. 6 mib expression is negatively regulated by Su(H)-dependent Notch activation. (A) mib expression in (a) wt and (b) mibta52b embryos at 22hpf, lateral view. The inserts show the mib expression in (a′–b′) the brain region, dorsal view and (a″–b″) a transverse section across posterior trunk. (B) her4 expression in (a–a′) control wt and (b–b′) su(h)-m7-injected embryos at 22hpf; (a–b) head region, dorsal view; (a′–b′) trunk region, lateral view. mib expression in (c) control wt and (d) su(h)-m7-injected embryos at 22hpf, lateral view. The inserts show the mib expression in the (c′–d′) head region, dorsal view and (c″–d″) a transverse section across posterior trunk. (C) her4 expression in (a–a′) control wt, (b–b′) DAPT-treated and (c–c′) X-Su(H)/Ank mRNA-injected embryos at 22hpf; (a–c) head region, dorsal view; (a′–c′) trunk region, lateral view. mib expression in (d–d′) control wt, (e–e′) DAPT-treated, and (f) X-Su(H)/Ank mRNA-injected embryos at 22hpf; (d–f) mid- and hindbrain region, dorsal view; (d′–e′) trunk and tail region, lateral view. (D) Coexpression of mib with ngn1 or dbx1a in hindbrain differentiating neurons at 42hpf. (a–c) and (e) are dorsal view; (d) and (f–h) are lateral view. Note that ngn1 and dbx1a are coexpressed with mib in different subpopulations of neurons.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dbx1a WT standard conditions High-pec hindbrain ISH
High-pec neuron ISH
her4.1 WT control 26+ somites diencephalon ISH
26+ somites hindbrain ISH
26+ somites midbrain ISH
26+ somites neural tube ISH
26+ somites presumptive neural retina ISH
WT standard conditions 26+ somites diencephalon ISH
26+ somites hindbrain ISH
26+ somites midbrain ISH
26+ somites neural tube ISH
26+ somites presumptive neural retina ISH
WT chemical treatment: DAPT 26+ somites Not Detected diencephalon ISH
26+ somites Not Detected hindbrain ISH
26+ somites Not Detected midbrain ISH
26+ somites Not Detected neural tube ISH
WT + MO4-rbpja,rbpjb standard conditions 26+ somites Not Detected diencephalon ISH
26+ somites Not Detected hindbrain ISH
26+ somites Not Detected midbrain ISH
26+ somites Not Detected neural tube ISH
mib1 WT control 26+ somites brain ISH
26+ somites neural tube ISH
WT standard conditions 26+ somites brain ISH
26+ somites neural tube ISH
High-pec hindbrain ISH
High-pec neuron ISH
WT chemical treatment: DAPT 26+ somites brain ISH
26+ somites neural tube ISH
WT + MO4-rbpja,rbpjb standard conditions 26+ somites brain ISH
26+ somites neural tube ISH
mib1ta52b/ta52b standard conditions 26+ somites brain ISH
26+ somites neural tube ISH
neurog1 WT standard conditions High-pec hindbrain ISH
High-pec neuron ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 305(1), Zhang, C., Li, Q., Lim, C.H., Qiu, X., and Jiang, Y.J., The characterization of zebrafish antimorphic mib alleles reveals that Mib and Mind bomb-2 (Mib2) function redundantly, 14-27, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.