ZFIN ID: ZDB-FIG-061130-2
Ochi et al., 2006 - Hhip regulates zebrafish muscle development by both sequestering Hedgehog and modulating localization of Smoothened. Developmental Biology   297(1):127-140 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Bud to 5-9 somites

Fig. 2 Cooperative activity of Hhip and Patched is required for restricted expression of myod in adaxial cells. (A–B) ptc1 is expressed in adaxial cells at bud stage in a control embryo (A) and is reduced by overexpression of hhip (B, arrow; 17/28 injected embryos). (C–F) In contrast, overexpression of hhip does not affect expression of wnt11, a marker of notochord, (C–D; 9/9 injected embryos) or dmrt2, a marker of paraxial mesoderm and somites (E–F, 13/14 injected embryos). (G–H) Overexpression of hhip inhibits myod expression. (G) myod is detected in adaxial cells and somites in a control embryo at the 8-somite stage. (H) myod expression is strongly reduced in a hhip mRNA-injected embryo (arrow, 25/46 injected embryos). (I–K) ptc1 expression is increased in a hhip-MO-injected embryo (I, arrow; 7/9 injected embryos). hhip-MO does not affect expression of wnt11 (J, 5/6 injected embryos) or dmrt2 (K, 11/11 injected embryos). (L–O) Reduction of Hhip and Ptc results in spread of myod expression laterally in the paraxial mesoderm. (L) Expression of myod in a control embryo. (M) Expression of myod is slightly upregulated in cells adjacent to adaxial cells in a hhip-MO-injected embryo (arrow, 23/93 injected embryos). (N) Injection of ptc-MO has a stronger effect than injection of hhip-MO (arrow, 21/27 injected embryos). (O) Reduction of both Hhip and Ptc causes a significant increase in myod expression in paraxial mesoderm (arrow, 27/27 injected embryos). Dorsal views, bud stage (A–D, I–J, L–O) and 8-somite stage (E–H, K), anterior towards the top. Scale bar: 200 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dmrt2a AB standard conditions Bud paraxial mesoderm ISH
AB + MO2-hhip + MO3-hhip standard conditions Bud paraxial mesoderm ISH
myod1 AB standard conditions Bud paraxial mesoderm ISH
5-9 somites adaxial cell ISH
5-9 somites somite ISH
AB + MO1-ptch1 + MO1-ptch2 standard conditions Bud paraxial mesoderm ISH
AB + MO1-ptch1 + MO1-ptch2 + MO2-hhip + MO3-hhip standard conditions Bud paraxial mesoderm ISH
AB + MO2-hhip + MO3-hhip standard conditions Bud paraxial mesoderm ISH
ptch2 AB standard conditions Bud paraxial mesoderm ISH
AB + MO2-hhip + MO3-hhip standard conditions Bud paraxial mesoderm ISH
wnt11f2 AB standard conditions Bud notochord ISH
AB + MO2-hhip + MO3-hhip standard conditions Bud notochord ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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Reprinted from Developmental Biology, 297(1), Ochi, H., Pearson, B.J., Chuang, P.T., Hammerschmidt, M., and Westerfield, M., Hhip regulates zebrafish muscle development by both sequestering Hedgehog and modulating localization of Smoothened, 127-140, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.