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ZIRC
ZFIN ID: ZDB-FIG-060726-9
Brand et al., 1996 - Mutations in zebrafish genes affecting the formation of the boundary between midbrain and hindbrain. Development (Cambridge, England)   123:179-190
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-5

Fig. 8 Expression of MHB markers is affected in ace embryos. Shown are lateral views of wild-type (left) and ace mutant (right) embryos. Arrows point to the posterior border of the tectum. (A,B) eng protein (dark brown staining around the MHB) is not detected in the tectum, tegmentum and cerebellum of 28 h ace mutant embryos. (C,D) pax-b expression is missing in the posterior midbrain of 24 h ace mutant embryos, but is unaffected elsewhere. (E,F) wnt1 expression is not found in the posterior tectum of 26 h ace mutant embryos. Notice the extension of the wnt1 stripe in the dorsal tectum to more posterior levels. Expression in the rhombomeres (arrowheads) is not affected. (G,H) Expression of Zash1A in the tectal ventricular zone (arrowhead) is strongly increased in the mutant embryo compared to its sibling at 26 h. Stainings of the embryos in G and H were done in the same vial. Scale bar, 90 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ascl1a WT standard conditions Prim-5 optic tectum ventricular zone ISH
fgf8ati282a/ti282a standard conditions Prim-5 optic tectum ventricular zone ISH
pax2a WT standard conditions Prim-5 midbrain posterior region ISH
fgf8ati282a/ti282a standard conditions Prim-5 Not Detected midbrain posterior region ISH
wnt1 WT standard conditions Prim-5 optic tectum posterior region ISH
fgf8ati282a/ti282a standard conditions Prim-5 Not Detected optic tectum posterior region ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-eng IHC WT standard conditions Prim-5 cerebellum
IHC Prim-5 optic tectum
IHC Prim-5 tegmentum
IHC fgf8ati282a/ti282a standard conditions Prim-5 Not Detected cerebellum
IHC Prim-5 Not Detected optic tectum
IHC Prim-5 Not Detected tegmentum
Phenotype Details
Fish Conditions Stage Phenotype
fgf8ati282a/ti282a standard conditions Prim-5 cerebellum aplastic, abnormal
Prim-5 midbrain hindbrain boundary aplastic, abnormal
Prim-5 optic tectum increased size, abnormal
Prim-5 tegmentum morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.