ZFIN ID: ZDB-FIG-060726-6
Brand et al., 1996 - Mutations in zebrafish genes affecting the formation of the boundary between midbrain and hindbrain. Development (Cambridge, England)   123:179-190
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: 5-9 somites to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage Range: 5-9 somites to Prim-5

Fig. 4 Expression of markers of the MHB in noi. Shown are dorsal (A-F) and lateral (G,H) views of wild type and noith44 mutant embryos, stained with markers of the MHB as indicated. (A,B) mAb4D9 staining for eng proteins at 28 h. Staining is eliminated in the MHB of mutant embryos. (C,D) Doubly stained embryos at the 8-somite stage, for krx20 RNA in blue and eng proteins in brown. No eng proteins are detected in the primordium of the MHB of mutant embryos. (E,F) Expression of wnt1 at the 20-somite stage posterior to the tectum (tec) is eliminated in noi, as is the posteriorly adjoining cerebellar fold. Notice the reduced distance between the wnt1-expressing domains in the dorsal forebrain and the first rhombomere (arrowheads). (G,H) zash1A staining at 26 h. A gap of staining (arrowheads) is observed at the MHB between the tegmental and rhombomeric expression domains, which is absent in the mutant embryo. Scale bar, 75 mm (A,B,G,H); 100 µm (C,D); 120 µm (E,F).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ascl1a WT standard conditions Prim-5 hindbrain ISH
Prim-5 tegmentum ISH
pax2ath44/th44 standard conditions Prim-5 hindbrain ISH
Prim-5 tegmentum ISH
egr2b WT standard conditions 5-9 somites presumptive rhombomere 3 ISH
5-9 somites presumptive rhombomere 5 ISH
pax2ath44/th44 standard conditions 5-9 somites presumptive rhombomere 3 ISH
5-9 somites presumptive rhombomere 5 ISH
wnt1 WT standard conditions 20-25 somites forebrain ISH
20-25 somites midbrain hindbrain boundary ISH
20-25 somites upper rhombic lip ISH
pax2ath44/th44 standard conditions 20-25 somites forebrain ISH
20-25 somites Not Detected midbrain hindbrain boundary ISH
20-25 somites upper rhombic lip ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-eng IHC WT standard conditions 5-9 somites midbrain hindbrain boundary neural rod
IHC Prim-5 midbrain hindbrain boundary
IHC pax2ath44/th44 standard conditions 5-9 somites Not Detected midbrain hindbrain boundary neural rod
IHC Prim-5 Not Detected midbrain hindbrain boundary
Phenotype Details
Fish Conditions Stage Phenotype
pax2ath44/th44 standard conditions 5-9 somites - Prim-5 midbrain hindbrain boundary aplastic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.