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ZFIN ID: ZDB-FIG-060328-12
Sumanas et al., 2006 - Ets1-Related Protein Is a Key Regulator of Vasculogenesis in Zebrafish. PLoS Biology   4(1):e10 Full text @ PLoS Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 5-9 somites to 20-25 somites

Fig. 5 Molecular Analysis of Early Vasculogenesis in etsrp Morphants (A, B, E, F, I, K) Uninjected control embryo; (C, D, G, H, J, L) 8–10 ng etsrp MO2-injected embryo. Anterior is to the left in all panels. (A–H) Embryos were flat mounted with their yolk removed. (A–D) scl expression; six-somite (A,C) and ten-somite (B,D) stages. Note that the anterior domain of scl expression (arrows) is reduced and the trunk domain (arrowheads) is missing in etsrp morphants. (E–H) fli1 expression; six-somite (E,G) and ten-somite (F,H) stages. Note that the anterior domain of fli1 expression (arrows) is missing in the etsrp morphants, while the posterior domain is not affected. Also note that the trunk domain of fli1 expression (arrowheads, F,H) is missing at the ten-somite stage in etsrp morphants. (I–L) etsrp knockdown blocks angioblast migration towards the midline as assayed by etsrp expression at the 16-somite (I,J) and 20-somite (K,L) stages. (I,K) Uninjected control embryo; (J,L) 7.5 ng etsrp MO2-injected embryo. Dorsal view, anterior is to the left. Note that the midline stripe of angioblasts (arrows) is missing in etsrp morphants. Also notice more intense etsrp expression in pre-migratory angioblasts (arrowheads) in etsrp morphants as compared to control embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
etv2 WT standard conditions 14-19 somites lateral mesoderm ISH
20-25 somites lateral mesoderm ISH
WT + MO2-etv2 standard conditions 14-19 somites lateral mesoderm ISH
20-25 somites lateral mesoderm ISH
fli1a WT standard conditions 5-9 somites anterior lateral mesoderm ISH
5-9 somites posterior lateral mesoderm ISH
10-13 somites anterior lateral mesoderm ISH
10-13 somites posterior lateral mesoderm ISH
WT + MO2-etv2 standard conditions 5-9 somites posterior lateral mesoderm ISH
10-13 somites posterior lateral mesoderm ISH
tal1 WT standard conditions 5-9 somites anterior lateral mesoderm ISH
5-9 somites posterior lateral mesoderm ISH
10-13 somites anterior lateral mesoderm ISH
10-13 somites posterior lateral mesoderm ISH
WT + MO2-etv2 standard conditions 5-9 somites posterior lateral mesoderm ISH
10-13 somites anterior lateral mesoderm ISH
10-13 somites posterior lateral mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Biol.