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ZFIN ID: ZDB-FIG-060328-11
Sumanas et al., 2006 - Ets1-Related Protein Is a Key Regulator of Vasculogenesis in Zebrafish. PLoS Biology   4(1):e10 Full text @ PLoS Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-5

Fig. 4 Molecular Analysis of Vascular Endothelial and Hematopoietic Markers in etsrp Morphants (A, C, E, G, I, K, M, O, Q, S) Uninjected control embryo; (D, H, J, L, P, R, T) 8 ng etsrp MO2-injected embryo; (B, F, N) 12 ng etsrp MO1+MO2 (1:1) mix-injected embryos for the maximum knockdown. All embryos are at 24 hpf, except as noted. Scale bar, 0.2 mm. (A,B) flk1 expression; (C,D) admr expression; (E,F) cdh5 expression; (G,H) dusp5 expression; (I,J) flt4 expression; (K,L) crl expression. Note that the vascular expression of the markers in (A–L) is almost absent in etsrp morphants. (M,N) fli1 expression; (O,P) etsrp expression. Note the more intense etsrp expression and reduced fli1 expression in angioblasts which remain dispersed and fail to coalesce into blood vessels in etsrp morphants. Inset, (P) DIC image of scattered angioblasts in etsrp morphants. (Q,R) scl expression, 22 hpf. Note that scl expression appears unaffected at this stage except for more intense staining in a subset of head vessels in etsrp morphants (arrowhead). (S,T) gata1 expression, 22 somites. Note that no significant difference in hematopoietic gata1 expression is observed between the control embryos and etsrp morphants.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cdh5 WT standard conditions Prim-5 blood vasculature ISH
WT + MO1-etv2 + MO2-etv2 standard conditions Prim-5 cranial vasculature ISH
clec14a WT standard conditions Prim-5 blood vasculature ISH
WT + MO2-etv2 standard conditions Prim-5 cranial vasculature ISH
dusp5 WT standard conditions Prim-5 blood vasculature ISH
WT + MO2-etv2 standard conditions Prim-5 cranial vasculature ISH
etv2 WT standard conditions Prim-5 blood vasculature ISH
WT + MO2-etv2 standard conditions Prim-5 blood vasculature ISH
fli1a WT standard conditions Prim-5 blood vasculature ISH
Prim-5 cranial vasculature ISH
WT + MO1-etv2 + MO2-etv2 standard conditions Prim-5 cranial vasculature ISH
flt4 WT standard conditions Prim-5 vein ISH
WT + MO2-etv2 standard conditions Prim-5 cranial vasculature ISH
gata1a WT standard conditions 20-25 somites intermediate cell mass of mesoderm ISH
WT + MO2-etv2 standard conditions 20-25 somites intermediate cell mass of mesoderm ISH
gpr182 WT standard conditions Prim-5 blood vasculature ISH
WT + MO2-etv2 standard conditions Prim-5 cranial vasculature ISH
kdrl WT standard conditions Prim-5 blood vasculature ISH
WT + MO1-etv2 + MO2-etv2 standard conditions Prim-5 cranial vasculature ISH
tal1 WT standard conditions 26+ somites intermediate cell mass of mesoderm ISH
26+ somites rostral blood island ISH
WT + MO2-etv2 standard conditions 26+ somites intermediate cell mass of mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Biol.