header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-060328-14
Sumanas et al., 2006 - Ets1-Related Protein Is a Key Regulator of Vasculogenesis in Zebrafish. PLoS Biology   4(1):e10 Full text @ PLoS Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-5

Fig. 7 etsrp Is Required for vegf and scl Signaling (A–D) Etsrp is required for Vegf signaling as assayed for flk1 expression at 26 hpf. (A) Control uninjected embryo, (B) vegf RNA-injected embryo, (C) 7.5 ng of etsrp MO2-injected embryo, (D) vegf RNA- and etsrp MO2-co-injected embryo. Note that vegf RNA induces strong flk1 expression in (B) while vegf RNA and etsrp MO co-injection results in loss of flk1 expression in (D), similar to the etsrp morphant phenotype in (C). (E,F) Etsrp expression analysis in Vegf morphants at 26 hpf. (E) Control uninjected embryo; (F) 10.5 ng of vegf MO-injected embryo. Note that vegf morphants have lost etsrp expression in the intersegmental vessels (arrowhead, E). (G–J) Scl knockdown affects gata1 but not etsrp expression at the 15-somite stage. Dorsal view, anterior is to the left. (G,I) Control uninjected embryo; (H,J) 10 ng scl UTR-MO-injected embryo. (G,H) gata1 expression; (I,J) etsrp expression. (K–N) Etsrp is required for scl signaling in clo mutants as analyzed for flk1 expression at the 15-somite stage. (K) Control uninjected embryo; (L) 7.5 ng etsrp MO2-injected embryo; (M) scl RNA-injected embryo; (N) scl RNA- and etsrp MO2-co-injected embryo. Note that scl RNA causes ectopic flk1 expression in (M) which is lost upon knockdown of Etsrp in (N).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
etv2 WT standard conditions 14-19 somites lateral mesoderm ISH
Prim-5 blood vasculature ISH
WT + MO1-vegfaa standard conditions Prim-5 caudal vein plexus ISH
Prim-5 Not Detected dorsal aorta ISH
WT + MO7-tal1 standard conditions 14-19 somites lateral mesoderm ISH
gata1a WT standard conditions 14-19 somites lateral mesoderm ISH
WT + MO7-tal1 standard conditions 14-19 somites Not Detected lateral mesoderm ISH
kdrl WT standard conditions 14-19 somites lateral mesoderm ISH
WT + MO2-etv2 standard conditions 14-19 somites Not Detected lateral mesoderm ISH
Prim-5 blood vasculature ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Biol.