ZFIN ID: ZDB-FIG-050607-10
Thorpe et al., 2005 - Wnt/β-catenin regulation of the Sp1-related transcription factor sp5l promotes tail development in zebrafish. Development (Cambridge, England)   132(8):1763-1772 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 4 wnt3a and wnt3a/wnt8 morphants lack mesodermal fates at the expense of floor plate in the caudal tail. All embryos were examined at approximately 27-28 hpf. More mildly affected wnt3a/wnt8 morphants that made some tail structures were selected for these analyses. (A-D) Tails of living 28 hpf embryos, anterior to the left. Arrows in B and D indicate premature termination of the notochord in wnt3a and wnt3a/wnt8 morphants, respectively, and the arrowhead in D marks enlarged neural tube lumen. (E-P) Embryos were fixed at 26 hpf and stained with the indicated marker. The caudal tip of the tail is outlined in black dashes for clarity where necessary. (E-H) Embryos stained with ntl to visualize the notochord, arrowheads indicate ntl expression in the tailbud; arrows in F and H indicate the tip of the truncated notochord. (I-L) α-tropomyosin staining to visualize the somites; arrowheads mark the posterior extent of somite formation. (M-P) Embryos stained for F-spondin to visualize the floor plate; arrow in P indicates the expanded floor plate in wnt3a/wnt8 embryos. (Q-T) Pan-neural ngn1 expression in the neural tube is unaffected by wnt3a and wnt8 inhibition.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
neurog1 AB standard conditions Prim-5 neural tube ISH
AB + MO1-wnt3a standard conditions Prim-5 neural tube ISH
AB + MO1-wnt3a + MO1-wnt8a + MO2-wnt8a standard conditions Prim-5 neural tube ISH
AB + MO1-wnt8a + MO2-wnt8a standard conditions Prim-5 neural tube ISH
spon1a AB standard conditions Prim-5 floor plate ISH
AB + MO1-wnt3a standard conditions Prim-5 floor plate ISH
AB + MO1-wnt3a + MO1-wnt8a + MO2-wnt8a standard conditions Prim-5 floor plate ISH
AB + MO1-wnt8a + MO2-wnt8a standard conditions Prim-5 floor plate ISH
tbxta AB standard conditions Prim-5 notochord ISH
Prim-5 tail bud ISH
AB + MO1-wnt3a standard conditions Prim-5 notochord ISH
Prim-5 tail bud ISH
AB + MO1-wnt3a + MO1-wnt8a + MO2-wnt8a standard conditions Prim-5 notochord ISH
Prim-5 tail bud ISH
AB + MO1-wnt8a + MO2-wnt8a standard conditions Prim-5 notochord ISH
Prim-5 tail bud ISH
tpma AB standard conditions Prim-5 somite ISH
AB + MO1-wnt3a standard conditions Prim-5 somite ISH
AB + MO1-wnt3a + MO1-wnt8a + MO2-wnt8a standard conditions Prim-5 somite ISH
AB + MO1-wnt8a + MO2-wnt8a standard conditions Prim-5 somite ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-wnt3a standard conditions Prim-5 notochord truncated, abnormal
Prim-5 somite decreased amount, abnormal
Prim-5 tail bud morphology, abnormal
AB + MO1-wnt3a + MO1-wnt8a + MO2-wnt8a standard conditions Prim-5 central canal dilated, abnormal
Prim-5 notochord truncated, abnormal
Prim-5 somite decreased amount, abnormal
Prim-5 tail bud bent, abnormal
Prim-5 tail bud morphology, abnormal
AB + MO1-wnt8a + MO2-wnt8a standard conditions Prim-5 tail bud morphology, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development