ZFIN ID: ZDB-FIG-050607-15
Thorpe et al., 2005 - Wnt/β-catenin regulation of the Sp1-related transcription factor sp5l promotes tail development in zebrafish. Development (Cambridge, England)   132(8):1763-1772 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: 1-4 somites

Fig. S2 The rate of cell proliferation in the tailbud is unchanged in wnt3a/wnt8 morphants. Embryos were injected with 2 mg/ml wnt3a MO (B), 0.5 mg/ml each of wnt8 ORF1 and ORF2 MOs (C), or wnt3a, wnt8 ORF1 and wnt8 ORF2 MOs together (D), and fixed at the 4-somite stage, with uninjected embryos as the control (A). tbx6 expression was visualized with a Fast Red substrate for the color reaction, which yields a fluorescent (red) product. Anti-phosphohistone H3 antibody marks mitotic nuclei (green). To determine the mitotic index, we calculated the number of mitotic cells within the tbx6 expression domain, divided by the total number of cells within that domain. Three embryos were scored for each group of embryos. We calculated a mitotic index of 7.5 (n=1281) for wild type, 6.7 (n=1089) for wnt3a morphants, 7.5 (n=772) for wnt8 MO embryos and 6.8 (n=772) for wnt3a/wnt8 morphants.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
tbx16l AB standard conditions 1-4 somites tail bud ISH
AB + MO1-wnt3a standard conditions 1-4 somites tail bud ISH
AB + MO1-wnt3a + MO1-wnt8a + MO2-wnt8a standard conditions 1-4 somites tail bud ISH
AB + MO1-wnt8a + MO2-wnt8a standard conditions 1-4 somites tail bud ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development