ZFIN ID: ZDB-FIG-050607-7
Thorpe et al., 2005 - Wnt/β-catenin regulation of the Sp1-related transcription factor sp5l promotes tail development in zebrafish. Development (Cambridge, England)   132(8):1763-1772 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: 10-13 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 10-13 somites to Long-pec

Fig. 1 wnt3a and wnt8 coordinately regulate tail development. (A-C) Wild-type development. (D-L) Embryos from a transgenic TOPdGFP line were injected with wnt3a MOs (D,E,F), wnt8 MOs (G,H,I) or both (J,K,L). Expression of the ß-catenin-responsive reporter was examined at bud stage by in situ hybridization with a probe for GFP (A,D,G,J). Embryos are shown in a dorsal view of the tailbud, with anterior to the top. At the 10-somite stage (B,E,H,K), TOPdGFP expression was again assessed by in situ hybridization, in this case with a Fast Red color reaction. myoD expression, to visualize the defect in tail development, is in blue. Embryos are flat mounted and shown in a dorsal view with anterior to the left. At 24 hpf (C,F,I,L), living embryos of each type are shown in a lateral view, anterior to the left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myod1 AB + MO1-wnt3a standard conditions 10-13 somites somite ISH
AB + MO1-wnt3a + MO1-wnt8a + MO2-wnt8a standard conditions 10-13 somites somite ISH
AB + MO1-wnt8a + MO2-wnt8a standard conditions 10-13 somites somite ISH
w25Tg standard conditions 10-13 somites somite ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-wnt3a standard conditions 10-13 somites tail bud decreased length, abnormal
Long-pec post-anal tail morphogenesis disrupted, abnormal
Long-pec post-vent region decreased length, abnormal
Long-pec whole organism decreased length, abnormal
AB + MO1-wnt3a + MO1-wnt8a + MO2-wnt8a standard conditions 10-13 somites tail bud decreased length, abnormal
Prim-5 post-anal tail morphogenesis disrupted, abnormal
Prim-5 post-vent region decreased length, abnormal
Prim-5 whole organism decreased length, abnormal
AB + MO1-wnt8a + MO2-wnt8a standard conditions 10-13 somites tail bud decreased length, abnormal
Prim-5 post-anal tail morphogenesis disrupted, abnormal
Prim-5 post-vent region decreased length, abnormal
Prim-5 whole organism decreased length, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development