Figure 1

Early expression of <italic>ntrk1</italic> is seen in the head and spinal cord using whole mount in situ hybridization at 16.5 hpf and 24 hpf.

(A and D) Expression of ntrk1 is seen at 16.5 hpf is seen in two sets of cranial ganglia (arrow in A and arrows in B). (B, C, E and F) At 24 hpf, expression is seen in two sets of cranial ganglia (arrows) as well as in the RB neurons (arrowheads). (A, B, C and F) Lateral view. (D and E) Dorsal view. All embryos are oriented with the head to the left. hb, marks the hindbrain, and ye, marks the yolk extension. Data are representative of three rounds of in situ hybridization with least 25 embryos per round.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-5
Figure 2

Early expression of <italic>ntrk2a</italic> is seen in the head and spinal cord using whole mount in situ hybridization at 16.5 hpf and 24 hpf.

Expression of ntrk2a is seen in the cranial ganglia (arrows) as well as in the RB neurons of the spinal cord (arrowheads) at (A and D) 16.5 hpf and (B, C, E and F) 24 hpf. The asterisk in E marks weak expression in the diencephalon that matches with previously published accounts. (A, B, C and F) Lateral view. (D and E) Dorsal view. All embryos are oriented with the head to the left. te, marks the telencephalon, mb, marks the midbrain, hb, marks the hindbrain, ov, marks the otic vesicle, chb, marks the caudal hindbrain and ye, marks the yolk extension. Data are representative of two rounds of in situ hybridization with at least 25 embryos per round.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-5
Figure 3

Early expression of p75 NTR (<italic>ngfrb</italic>) is seen in two sets of cranial ganglia using whole mount in situ hybridization at 24 hpf.

(A and C) Expression of ngfrb was not detected at 16.5 hpf and the staining that can be seen was non-specific (Fig. S2). (B, D and E) At 24 hpf, expression of ngfrb was seen in two sets of cranial ganglia (arrows). (A, B and E) Lateral view. (C and D) Dorsal view. All embryos are oriented with the head to the left. d, marks the diencephalon, cb, marks the cerebellum, hb, marks the hindbrain, chb, marks the caudal hindbrain and ye, marks the yolk extension. Data are representative of the results from two rounds of in situ hybridization with at least 25 embryos per round.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage Range: 14-19 somites to Prim-5
Figure 4

Early expression of <italic>ntrk2b</italic> is seen in the head using whole-mount in situ hybridization at 24 hpf.

(A and C) Expression of ntrk2b was not detectable at 16.5 hpf. (B, D and E) At 24 hpf, expression of ntrk2b is seen in the telencephalon (arrows). (A, B and E) Lateral view. (C and D) Dorsal view. e, marks the eye, d, marks the diencephalon, mhb, marks the midbrain-hindbrain boundary, and ye, marks the yolk extension. Data are representative of three rounds of in situ hybridization with at least 25 embryos per round.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage Range: 14-19 somites to Prim-5
Figure 5

Early expression of <italic>ntrk3a</italic> is seen in the head and spinal cord using whole-mount in situ hybridization at 24 hpf.

(A and C) Expression of ntrk3a was not detected at 16.5 hpf. (B, D and E) At 24 hpf, expression was seen in the telencephalon (arrow) and spinal cord (arrowheads). (A, B and E) Lateral view. (C and D) Dorsal view. All embryos are oriented with the head to the left. e, marks the eye, d, marks the diencephalon, and ye, marks the yolk extension. This data is representative of the results from three rounds of in situ hybridization with at least 25 embryos per round.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-5
Figure 6

Early expression of <italic>ntrk3b</italic> is seen throughout the head at 24 hpf using whole mount in situ hybridization.

(A and C) At 16.5 hpf, no expression was detected. (B and D) Expression was seen in the telencephalon (black arrows), the diencephalon (white arrows), the midbrain (red arrows), the hindbrain (blue arrows), and otic vesicle (black arrowhead). (A and B) Lateral view. (C and D) Dorsal view. e, marks the eye, cb, marks the cerebellum, and chb, marks the caudal hindbrain. All embryos are oriented with the head to the left. This data is representative of the results of three rounds of in situ hybridization with at least 25 embryos per round.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-5
Acknowledgments
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