Pax6 is essential for the establishment of the mid-diencephalon in zebrafish. (A-F) Whole mount double in situ hybridization (ISH) for indicated markers of 28 hours post-fertilization (hpf) zebrafish embryos of indicated experimental procedure. Double red arrows mark MDO thalamic primordial. In B, D and F, black dashed line marks the border between MDO and the prethalamus, and white dashed line marks the border between MDO and thalamus. (G) Schematic representation of a sagittal section of zebrafish forebrain at 28 hpf. cTh, caudal thalamus; ETh, epithalamus; MDO, mid-diencephalic organizer; PrT, pretectum; PTh, prethalamus; rTh, rostral thalamus; ZLI, zona limitans intrathalamica.

Pax6 regulates the expression domain of shh expression in the MDO in zebrafish. (A-H) Lateral views of wholemount ISH for shh in 28 hpf zebrafish embryos of the indicated experimental procedure. (A-D) red arrows mark the MDO, whereas red arrowheads mark the position of the reduced MDO. (A′,B′,C′,D′) show a coronal section of the prosencephalon with anterior to the bottom. hpf, hours post-fertilization; ISH, in situ hybridization; MDO, mid-diencephalic organizer.

EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-5

Pax6 controls development of the epithalamus via regulation of MDO Shh function in zebrafish. (A-L′) Lateral and dorsal views of wholemount ISH for otx5 (A-F′) and cxcr4b (G-L′) in 28 hpf zebrafish embryos of the indicated experimental procedure. Red arrows marks the cxcr4b positive habenula precursor cells. hpf, hours post-fertilization; ISH, in situ hybridization; MDO, mid-diencephalic organizer.

Acknowledgments
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