Fig. 2

Relative expression of zebrafish genes after CPAs exposure (CPAs) and cryopreservation (Cryo).

Cryopreservation affects gene expression levels in zebrafish genital ridges. Analysis was carried out in zebrafish genital ridges (GRs) containing primordial germ cells (PGCs). Expression levels for each gene relative to actb2 were calculated for all samples using the 2-ΔΔCt method. All results were expressed as the means ± SE of the 2-ΔΔCt method of three independent experiments with three replicates for each. The Student’s t-test (µ=1) was performed to identify changes in gene expression levels after cryopreservation. Asterisks showed significant (p< 0.05) downregulation after cryopreservation (Cryo).

EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: 26+ somites
Acknowledgments
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