Fig. 4

Fig. 4

AP2M1 confers stemness to hepatocytes. A The scatter plots depict the relationship between AP2M1 expression levels and SOX2 and POU5F1 expression levels. The blue line represents the linear regression fit with the shaded area indicating the 95% CI. BAP2M1 siRNA decreased the expression levels of DLK-1, Nanog, IGF-2, CK-19, SOX-2, SOX-9, SALL-4, EPCAM, AFP and PROM-1 in HepG2 cells as analyzed by qPCR. Data were quantified and expressed as the mean ± SEM of 5–6 independent experiments. *p < 0.05 and ***p < 0.001 vs. value in the negative control. C HepG2 cells were stained with crystal violet staining using the Matrigel invasion assay as described. Data were quantified and expressed as the mean ± SEM of 10 independent experiments. ***p < 0.001 vs. value in the negative control. D Expression of dlk1, nanog, igf2a, keratin93, sox2, sox9, sall4, and prom1a in 48hpf zebrafish embryos was detected by RT-PCR and samples were prepared in triplicates. Particularly, dlk1, nanog, and prom1a were decreased by **p < 0.01, ***p < 0.001, *p < 0.05, respectively