Figure 1

Figure 1

Zebrafish sirt7 is suppressed by hypoxia.A, quantitative real-time PCR analysis (qPCR) of sirt7, as well as well-defined hypoxia-responsive genes, including cited2, pai1, phd3, vegfaa, and epoa, in zebrafish larvae (3 dpf) under normoxia (Nor) and hypoxia (Hyp). B, qPCR analysis of sirt7, as well as well-defined hypoxia-responsive genes, including phd3, cited2, ldha, and vegfaa, in ZFL cells under normoxia (Nor) and hypoxia (Hyp). C, qPCR analysis of sirt7 in ZFL cells transfected with empty vector control (Flag empty) or Flag-hif-1αa, or Flag-hif-1αb, or Flag-hif-2αa, or Flag-hif-2αb under normoxia. D, qPCR analysis of phd3 in ZFL cells transfected with empty vector control (Flag empty) or Flag-hif-1αa, or Flag-hif-1αb, or Flag-hif-2αa, or Flag-hif-2αb under normoxia. E, qPCR analysis of cited2 in ZFL cells transfected with empty vector control (Flag empty) or Flag-hif-1αa, or Flag-hif-1αb, or Flag-hif-2αa, or Flag-hif-2αb under normoxia. F, qPCR analysis of ldha in ZFL cells transfected with empty vector control (Flag empty) or Flag-hif-1αa, or Flag-hif-1αb, or Flag-hif-2αa, or Flag-hif-2αb under normoxia. G, qPCR analysis of vegfaa in ZFL cells transfected with empty vector control (Flag empty) or Flag-hif-1αa, or Flag-hif-1αb, or Flag-hif-2αa, or Flag-hif-2αb under normoxia. p Values were calculated by unpaired Student's t test (A and B) or two-way ANOVA analysis (C–G); ns, not significant, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 and ∗∗∗∗p < 0.0001; data based on one representative experiment performed in three biological replicates from at least three independent experiments (mean ± SD).