IMAGE

Figure 5

ID

ZDB-IMAGE-210923-29

Genes

Publication

Li et al., 2021 - Developmental Angiogenesis Requires the Mitochondrial Phenylalanyl-tRNA Synthetase

All Figures

Figures for Li et al., 2021

Image

Figure Caption

Figure 5

The deficiency of FARS2 impairs angiogenesis by disrupting the Notch and Wnt signaling pathways. (A) The expression levels of genes involved in the Notch/Wnt pathways in control and fars2 zebrafish morphants, as determined by qRT-PCR analyses (n = 6–10 individual embryos). ^***P < 0.001, ^**P < 0.01, ^*P < 0.05; ns, not significant. (B) The relative mRNA expression levels of Notch/Wnt pathway-related genes. The human umbilical vein endothelial cells (HUVECs) were transfected with the indicated siRNAs for 48 h and then harvested for qRT-PCR analysis. The measurements were made in triplicate (mean and SEM), and the results are indicative of three independent experiments. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001. (C) Western blot analyses of NOTCH1 and β-catenin protein levels. The HUVECs were transfected with the indicated siRNAs for 48 h prior to analysis. (D) Quantification of the western blotting data described in (C). The measurements were made in triplicate (mean and SEM). ^*P < 0.05, ^**P < 0.01.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cardiovasc Med