ZFIN Image: Zhao et al., 2019, Fig. S3

Image

Figure Caption

Fig. S3

sas10 is essential for the organogenesis of digestive organs.

(A) Whole-mount in situ hybridization (WISH) using the sas10 probe on WT unfertilized 2 eggs and embryos at 6hpf, 12hpf, and 1dpf to 5dpf. (B) Upper panel: diagram showing the sas10 genomic DNA. Red bar, exons; blue line, introns. Lower panels: showing the DNA sequence changes in sas10Δ8 and sas10Δ2 two mutant alleles which are mutated in the exon4 of the sas10 gene (red letters, deletion; blue letters, insertion; small letters: intron). (C) Representative images showing the overall morphology of a 5dpf-old WT and sas10Δ2 (sΔ2). Black arrow, cardiac region; red arrow, swimming bladder; blue arrow, eye. (D-G) WISH using the prox1 (D) and hhex (E) probes on 30hpf- and 50hpf-old embryos, and the gata6 (F) and foxa3 (G) probes on 30hpf-, 50hpf- and 3dpf-old embryos as shown. The number of total embryos examined (as denominator) and the number of embryos exhibiting the displayed phenotype (as numerator) are shown at the bottom.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nucleic Acids Res.