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ZDB-IMAGE-130730-28

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van der Vaart et al., 2013 - Functional analysis of a zebrafish myd88 mutant identifies key transcriptional components of the innate immune system

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Fig. 7 qPCR analysis of the response of myd88^-/- and wild-type embryos to M. marinum infection.
Expression of il1b, tnfa, il8, cxcl-c1c, mmp9 and ifnphi1 was analyzed by qPCR in M. marinum Mma20-infected wild-type (Wt) and mutant (Mu) embryos. RNA samples from M. marinum-infected embryos (inf) and uninfected controls were taken at 4 dpi. Each data point represents an individual embryo and lines indicate the mean relative expression level, with values for uninfected wild type set at 1. Significant differences were calculated by one-way ANOVA with Tukey’s multiple comparison method as a post-hoc test; *P<0.05, ***P<0.001; ns, not significant.

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