Fig. 8

Fig. 8

Expression of endoderm markers is reduced in MZeomesa embryos. (A-C′, G, G′, I-J′) lateral views, (D-F′, H, H′, K-M′) animal pole views of embryos stained by whole mount in situ hybridzation. (A-B′) mxtx2 expression at sphere (A-A′′) and dome stage. (B′) gsc in red marks dorsal. (C-D′′) ndr1 expression at sphere (arrow, C,C′) and 40% epiboly (D-D′′). (E, E′) ndr2 expression at 40% epiboly. (F,F′) lft1 expression at 40% epiboly (G-H′) sox32 expression at 40% epiboly, (H′) gsc in red marks dorsal. sox32 expression (I,I′) and sox17 expression (J,J′) at 75% epiboly. Arrowheads indicate dorsal forerunner cells. (K-K′′) gata5 expression at 40% epiboly. (L,L′) bon expression at 40% epiboly (M,M′) og9x expression at 40% epiboly. (N.N′) pou5f1 at oblong/sphere. Genotypes indicated in lower left, probe in upper right and percentages of embryos that exhibited given phenotype shown in lower right where appropriate.