FIGURE

Fig. 4 - Supplemental 1

ID

ZDB-FIG-260520-46

Publication

Wafer et al., 2026 - A quantitative in vivo CRISPR-imaging platform identifies regulators of hyperplastic and hypertrophic adipose morphology in zebrafish

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Fig. 4 - Supplemental 1

Image-based adipose morphology profiling detects hypertrophic lipid droplet (LD) expansion in gh1 CRISPR mutants. (a) Nile Red fluorescence images showing adiposity in a representative Cas9-only control (top) and gh1 F0 CRISPR mutant (bottom) at ~1 month of age. Black signal denotes Nile Red-positive neutral lipid. Dotted line indicates operculum. (b) High-magnification images of subcutaneous adipose tissue (SAT) from Cas9-only control and gh1 CRISPant zebrafish. Cyan outlines show automated segmentation of individual LDs. Scale bar is 200 µm. (c) Kernel density plot of morphology values for gh1 mutants (n=9, cyan) compared to baseline wild-type fish (n=194, grey). Morphology values were significantly increased in gh1 mutants (Kolmogorov-Smirnov test, p=0.04), indicating a shift towards hypertrophic morphology.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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