Fig. 2

Prioritisation of candidate adipose morphology genes based on conservation to zebrafish and expression dynamics during progenitor differentiation. (a) Gene Ontology (GO) term enrichment analysis of the 102 candidate morphology genes enriched in adipose stem and progenitor cells. Four GO terms related to development and differentiation were significantly enriched, encompassing 54 genes. (b) Venn diagram showing gene overlap between the enriched GO terms. Cell differentiation (GO:0030154) and cellular developmental process (GO:0048869) had 100% overlap. (c) Protein conservation and expression during adipocyte progenitor differentiation for each candidate gene. Grey bars denote percentage protein similarity between human and zebrafish orthologues. Dot plots show scaled mean expression (colour) and percentage of cells expressing each gene (dot size) across four cell populations identified during in vitro progenitor differentiation: non-induced progenitors, MGP+ SWAT cells, ADIPOQ+ adipogenic cells and others (Yang Loureiro et al., 2023). Genes are ordered by protein similarity. (d) PCA projection of single cells coloured by cluster designation from Yang Loureiro et al., 2023. (e) Expression of selected candidate morphology genes projected onto the PCA embedding, illustrating enrichment during specific stages of adipocyte differentiation.