FIGURE

Figure 6

ID

ZDB-FIG-260427-41

Publication

Choi et al., 2026 - 3D imaging with enhanced transparency, signal-to-background ratios, and antigen detection using HyPer-3D

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Figure 6

3D interrogation of muscular organs with enhanced clarity

(A–E) IF performed on whole atria for HCN4 (red) that marks the sinoatrial node (SAN). Atria processed using standard H₂O₂ treatment (control, Cont) or HyPer-3D (HyP) were imaged side-by-side for comparison (A; scale bars, 2 mm, RA, right atria; LA, left atria). High-magnification analyses of bracketed regions in (A) showed a distinct HCN4⁺ node at the terminal crest (TC) of hearts imaged with HyPer-3D (D, coronal view; E, lateral view), which was not readily discernible in control hearts (B, coronal view; C, lateral view). Scale bars, 0.25 mm.

(F) 3D confocal microscopy imaging of formamide/glycerol-cleared whole atria immunolabeled for both the working myocardium (cTROPONIN-T, green) and HCN4 pacemaker channels (red; scale bars, 1 mm). Inset shows SAN alone.

(G and H) Staining with DAPI and HCN4 (G, merged; H, HCN4 alone; scale bars, 0.2 mm).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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