FIGURE

Fig. 5

ID

ZDB-FIG-260203-12

Publication

Herget et al., 2025 - Pth4 neurons define a novel hypothalamic circuit that promotes sleep via brainstem monoaminergic neurons

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Fig. 5

Sleep induced by optogenetic stimulation of Pth4 neurons is suppressed in dbh mutant fish (A and B) Pth4 neuron fibers (A, tdTomato) and presynaptic terminals (B, synaptophysin-TagRFP [SypRFP]) are found in close proximity to the somata and neurites of GFP-labeled LC neurons. (C–C″) HCR for dbh, which labels LC neurons, and pth2rb shows that pth2rb is expressed in LC neurons (co-expression in 190/199 cells, n = 18 fish). Scale bars, 50 μm. (D–G) Optogenetic stimulation of Pth4 neurons suppresses locomotor activity and increases sleep in dbh+/+ fish (D and E) but not in dbh−/− fish (F and G) compared with non-transgenic siblings. Mean ± SEM line plots and Tukey boxplots are shown. n = number of fish. ∗∗∗∗p < 0.0001 by Mann-Whitney U test. See also Figures S2 and S4.

Expression Data

Genes:	dbh EGFP pth2rb TagRFP Tomato
Fish:	AB/TL ct821Tg/ct821Tg; ct833Tg/ct833Tg ct821Tg/ct821Tg; ct880Tg/ct880Tg
Anatomical Terms:	locus coeruleus locus coeruleus neuron locus coeruleus presynapse
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ct878Tg/+ ct878Tg/ct878Tg dbh^ct806/ct806; ct878Tg/+ dbh^ct806/ct806; ct878Tg/ct878Tg
Condition:	lighting conditions
Observed In:	locomotory behavior sleep homeostasis
Stage:	Day 4

Phenotype Detail

Acknowledgments

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