FIGURE

Fig. 3

ID

ZDB-FIG-260203-10

Publication

Herget et al., 2025 - Pth4 neurons define a novel hypothalamic circuit that promotes sleep via brainstem monoaminergic neurons

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Fig. 3

Sleep induction by optogenetic stimulation of QRFP/Pth4 neurons requires pth4 but not qrfp (A–D) Optogenetic stimulation of QRFP/Pth4 neurons suppresses locomotor activity and increases sleep in qrfp+/− (A and B) and qrfp−/− fish (C and D) compared with non-transgenic siblings. (E) FISH shows that pth4 and qrfp are co-expressed in the same hypothalamic neurons (586/589 cells, n = 23 fish). Scale bar, 25 μm. (F–I) Optogenetic stimulation of QRFP/Pth4 neurons suppresses locomotor activity and increases sleep in pth4+/+ fish (F and G) but not in pth4−/− fish (H and I) compared with non-transgenic siblings. Mean ± SEM line plots and Tukey boxplots are shown. n = number of fish. ∗∗∗∗p < 0.0001, ns, p > 0.05 by Mann-Whitney U test. See also Figures S2 and S3.

Expression Data

Genes:	pth4 qrfp
Fish:	AB/TL
Anatomical Term:	hypothalamus neuron
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pth4^iim21/+; ct878Tg/+ pth4^iim21/+; ct878Tg/ct878Tg pth4^iim21/iim21; ct878Tg/+ pth4^iim21/iim21; ct878Tg/ct878Tg qrfp^ct815/+; ct878Tg/+ qrfp^ct815/+; ct878Tg/ct878Tg qrfp^ct815/ct815; ct878Tg/+ qrfp^ct815/ct815; ct878Tg/ct878Tg
Condition:	lighting conditions
Observed In:	locomotory behavior sleep homeostasis
Stage:	Day 4

Phenotype Detail

Acknowledgments

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