ZFIN Image: Herget et al., 2025, Fig. 5

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Figure Caption

Fig. 5 Sleep induced by optogenetic stimulation of Pth4 neurons is suppressed in dbh mutant fish (A and B) Pth4 neuron fibers (A, tdTomato) and presynaptic terminals (B, synaptophysin-TagRFP [SypRFP]) are found in close proximity to the somata and neurites of GFP-labeled LC neurons. (C–C″) HCR for dbh, which labels LC neurons, and pth2rb shows that pth2rb is expressed in LC neurons (co-expression in 190/199 cells, n = 18 fish). Scale bars, 50 μm. (D–G) Optogenetic stimulation of Pth4 neurons suppresses locomotor activity and increases sleep in dbh+/+ fish (D and E) but not in dbh−/− fish (F and G) compared with non-transgenic siblings. Mean ± SEM line plots and Tukey boxplots are shown. n = number of fish. ∗∗∗∗p < 0.0001 by Mann-Whitney U test. See also Figures S2 and S4.

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Acknowledgments

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