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Fig. 3

ID

ZDB-FIG-250926-30

Publication

Liang et al., 2025 - Creation of colorless transparent tilapia using CRISPR/Cas9 mediated multi-gene mutation

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Fig. 3

Iridophores were largely recovered in pnp4a−/− mutants during the late stages of development. (A-F) At 35 dpf, the iris (B) and operculum (C)were covered with iridophores on wild-type fish (A), while only a small number of iridophores were observed on the iris (E) and operculum (F) of pnp4a−/− mutants (D), and 7–9 black vertical bars were formed in both wild-type fish and mutants. (G-L) At 90 dpf, both wild-type fish and mutants were covered with iridophores on the body surface (G, J) and iris (H, K) due to the recovery of iridophores in mutants. The wild-type fish displayed 7–9 clear black vertical bars (G), while the mutant fish displayed blurry vertical bars (J) on their body surface. Blue arrowheads indicate iridophores. Scale bar in A-F, 1 mm; scale bar in G, J, 1 cm; scale bar in H, K, 2 mm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from New biotechnology, 89, Liang, G., Lu, B., Dai, S., Li, M., Yao, J., Liu, H., Liu, X., Liu, X., Wang, D., Creation of colorless transparent tilapia using CRISPR/Cas9 mediated multi-gene mutation, 163-176, Copyright (2025) with permission from Elsevier. Full text @ N. Biotechnol.