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Fig. 2

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ZDB-FIG-250926-29

Publication

Liang et al., 2025 - Creation of colorless transparent tilapia using CRISPR/Cas9 mediated multi-gene mutation

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Fig. 2

No iridophores were observed in pnp4a−/− mutants at 4 and 10 dpf. A large number of iridophores were observed on the iris but not on the body surface of wild-type fish (A, B), while no iridophores were observed on the iris and body surface of the pnp4a−/− mutants (C, D) at 4 dpf. Many iridophores were observed on the iris, operculum, peritoneum and swim bladder of wild-type fish (E-G), while no iridophores were observed on the iris, yolk sac and body surface of pnp4a−/− mutants (H-J) at 10 dpf. Compared with the wild type fish, pnp4a−/− mutants displayed black iris (color of RPE) due to the loss of visible iridophores. Blue arrowheads indicate iridophores. Scale bar in A-J, 1 mm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from New biotechnology, 89, Liang, G., Lu, B., Dai, S., Li, M., Yao, J., Liu, H., Liu, X., Liu, X., Wang, D., Creation of colorless transparent tilapia using CRISPR/Cas9 mediated multi-gene mutation, 163-176, Copyright (2025) with permission from Elsevier. Full text @ N. Biotechnol.