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Fig. 1

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ZDB-FIG-250926-28

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Liang et al., 2025 - Creation of colorless transparent tilapia using CRISPR/Cas9 mediated multi-gene mutation

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Fig. 1

tyrb−/−;csf1ra−/− double mutants lacked melanin, xanthophores, and erythrophores. (A-P) Phenotype of wild-type fish, tyrb−/− and csf1ra−/− single mutants, and tyrb−/−;csf1ra−/− double mutants at 180 dpf. (A-D) wild-type fish have pigmented melanophores, xanthophores, erythrophores, and iridophores, with 7–9 black vertical bars covering the surface of the body. (E-H) tyrb−/− mutants displayed albino phenotype with no pigmented melanophores. (I-L) csf1ra−/− mutants displayed grey body color phenotype with no black vertical bars due to even distribution of melanophores and complete loss of xanthophores and erythrophores. (M-P) tyrb−/−;csf1ra−/− double mutants displayed silvery body color phenotype due to complete loss of melanin, xanthophores and erythrophores. The black, red, and orange arrows indicate melanophores, erythrophores, and xanthophores in skin (C, G, K, O) and pectoral fins (D, H, L, P), respectively. WT, wild-type fish; dpf, days post fertilization; scale bar in whole fish (A, D, G, J), 2 cm; scale bar in eyes (B, E, H, K), 2 cm; scale bar in skin (C, G, K, O) and pectoral fins (D, H, L, P), 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from New biotechnology, 89, Liang, G., Lu, B., Dai, S., Li, M., Yao, J., Liu, H., Liu, X., Liu, X., Wang, D., Creation of colorless transparent tilapia using CRISPR/Cas9 mediated multi-gene mutation, 163-176, Copyright (2025) with permission from Elsevier. Full text @ N. Biotechnol.