FIGURE

Figure 3

ID

ZDB-FIG-250826-33

Publication

Masiero et al., 2025 - Combined antiresorptive and new anabolic drug approach in osteogenesis imperfecta zebrafish models

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Figure 3

Analysis of osteoclast activity in Chi/+ and p3h1^−/− zebrafish. (A, B) TRAP staining quantification on haemal and neural arches and autocentrum of WT and Chi/+ (i) and Chi/+ untreated and treated with 4PBA, ALN, and 4PBA + ALN (ii). (C) Representative images of TRAP-stained WT and Chi/+ caudal vertebrae. (D, E) TRAP staining quantification on arches and vertebrae of WT and p3h1^−/− (i) and p3h1^−/− untreated and treated with 4PBA, ALN, and 4PBA + ALN (ii). (F) Representative images of TRAP-stained WT and p3h1^−/− caudal vertebrae. Red arrows indicate TRAP signal on vertebral bodies. The yellow asterisks indicate the intervertebral disc. Scale bar: 50 μm. In the graphs, the percentage of TRAP+ vertebrae on total number of analyzed vertebrae is reported. ^*p < .05, ^**p < .01, ^***p < .001, ^****p < .0001. Abbreviations: ALN, alendronate; NC, notochord; TRAP, tartrate-resistant acid phosphatase; 4PBA, 4-phenylbutyrate.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	p3h1^upv2/upv2 col1a1a^dc124/+
Conditions:	chemical treatment by environment: 4-phenylbutyrate chemical treatment by environment: 4-phenylbutyrate chemical treatment by environment: alendronic acid
Observed In:	hemal arch acid phosphatase activity neural arch acid phosphatase activity vertebra acid phosphatase activity
Stage:	Adult

Phenotype Detail

Acknowledgments

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