Figure 3

Figure 3

Analysis of osteoclast activity in Chi/+ and p3h1^−/− zebrafish. (A, B) TRAP staining quantification on haemal and neural arches and autocentrum of WT and Chi/+ (i) and Chi/+ untreated and treated with 4PBA, ALN, and 4PBA + ALN (ii). (C) Representative images of TRAP-stained WT and Chi/+ caudal vertebrae. (D, E) TRAP staining quantification on arches and vertebrae of WT and p3h1^−/− (i) and p3h1^−/− untreated and treated with 4PBA, ALN, and 4PBA + ALN (ii). (F) Representative images of TRAP-stained WT and p3h1^−/− caudal vertebrae. Red arrows indicate TRAP signal on vertebral bodies. The yellow asterisks indicate the intervertebral disc. Scale bar: 50 μm. In the graphs, the percentage of TRAP+ vertebrae on total number of analyzed vertebrae is reported. ^*p < .05, ^**p < .01, ^***p < .001, ^****p < .0001. Abbreviations: ALN, alendronate; NC, notochord; TRAP, tartrate-resistant acid phosphatase; 4PBA, 4-phenylbutyrate.