FIGURE

Fig. 4

ID

ZDB-FIG-250818-36

Publication

Kawasaki et al., 2025 - Meioc-Piwil1 complexes regulate rRNA transcription for differentiation of spermatogonial stem cells

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Fig. 4

Meioc binds with Piwil1 and affects the localization of Piwil1. (A) Immunostaining of Ddx4 and Piwil1, Piwil2, Tdrd1, and Tdrd6a in wild-type and meiocmo/mo spermatogonia. The arrowhead: the Piwil1 signal in the nucleolus. (B) Immunostaining against Piwil1 and fibrillarin (left panels) and quantification of nucleolar Piwil1 (right panel) in wild-type and meiocmo/mo spermatogonia. Arrowheads: fibrillarin positive nucleolus. (C) Immunostaining of Piwil1 and Ddx4 (left panels) and quantification of Piwil1 in germ granules (right panel) in wild-type and meiocmo/mo spermatogonia. Arrowheads: Ddx4 positive germ granules. (D) Co-immunoprecipitation of Meioc and Piwil1 using testis lysate. Meioc signals were detected in Piwil1 immunoprecipitate and vice versa. Benzonase: addition of benzonase nuclease. **p<0.01. Scale bars: 10 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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